Human Albumin ELISA Kit (ab227933)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman Albumin ELISA Kit
See all Albumin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Serum 9 4% Inter-assay Sample n Mean SD CV% Serum 3 12.6% -
Sample typeUrine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
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Assay typeSandwich (quantitative)
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Sensitivity30 pg/ml
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Range312.5 pg/ml - 20000 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Urine 104 101% - 107% Serum 117 106% - 133% Heparin Plasma 111 104% - 116% EDTA Plasma 115 108% - 121% Citrate Plasma 112 106% - 117% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Human
Does not react with: Mouse, Rat, Cow, Rhesus monkey -
Product overview
Albumin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin levels in human plasma, serum, and urine.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binding about 80% of all plasma zinc.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformPre-coated microplate (12 x 8 well strips)
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Human Albumin Capture Antibody 10X 1 x 600µl Human Albumin Detector Antibody 10X 1 x 600µl Human Albumin Lyophilized Purified Protein 2 vials Antibody Diluent CP 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMB Development Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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Tissue specificityPlasma.
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Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
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Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
Applications
Our Abpromise guarantee covers the use of ab227933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of human Albumin standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Albumin in human serum and plasma samplesThe concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:5 million, plasma (citrate) 1:5 million, plasma (heparin) 1:5 million, and plasma (EDTA) 1:10 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 39.3 mg/mL in serum, 34.4 mg/mL in plasma (citrate), 30.8 mg/mL in plasma (heparin), and 34.5 mg/mL in plasma (EDTA).
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Interpolated concentrations of native Albumin in human urine samplesThe concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6,290 ng/mL in urine.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 33.31 mg/mL with a range of 22.5 – 46.2 mg/mL.
Protocols
References
ab227933 has not yet been referenced specifically in any publications.
