Key features and details
- Sensitivity: 1.875 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Alkaline Phosphatase ELISA Kit
See all Alkaline Phosphatase kits
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeSerum, Plasma, Other biological fluids, Tissue Homogenate
Assay typeSandwich (quantitative)
Sensitivity< 1.875 ng/ml
Range3.125 ng/ml - 200 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.
This Human Alkaline Phosphatase Kit (ab285249, E4572) is a sandwich ELISA assay for the quantitative measurement of ALP in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of ALP captured from the samples
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin/Detection antibody (Concentrated) 1 x 120µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 2 vials Micro ELISA Plate 1 unit Plate Sealer 5 units SABC dilution buffer 1 x 20ml Sample / Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase, germ cell
- Alkaline phosphatase, testicular and thymus
ab285249 has not yet been referenced specifically in any publications.