RabMAb

Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free (ab244036)

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Overview

  • Product name

    Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free
    See all Alpha 1 microglobulin kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Range

    1.56 ng/ml - 100 ng/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human Alpha-1-Microglobulin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab226899), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email to technical support with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human Alpha-1-Microglobulin Capture Antibody 1 x 100µg
    Human Alpha-1-Microglobulin Detector Antibody 1 x 100µg

  • Research areas

  • Function

    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.

  • Tissue specificity

    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.

  • Sequence similarities

    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.

  • Post-translational
    modifications

    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • A1M
    • Alpha 1 microglobulin/bikunin precursor
    • Alpha 1 microglycoprotein
    • Alpha-1 microglycoprotein
    • Alpha-1-microglobulin
    • AMBP
    • AMBP_HUMAN
    • Bikunin
    • Complex-forming glycoprotein heterogeneous in charge
    • EDC1
    • Growth inhibiting protein 19
    • HCP
    • HI-30
    • HI30
    • IATIL
    • ITI
    • ITI-LC
    • ITIL
    • Microglobulin (alpha 1)
    • Protein AMBP
    • Protein HC
    • Trypstatin
    • Uristatin
    • Uronic-acid-rich protein
    • UTI
    see all

  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab244036 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.

    References

    ab244036 has not yet been referenced specifically in any publications.

    Publishing research using ab244036? Please let us know so that we can cite the reference in this datasheet.

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