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Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free (ab244036)

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Sandwich ELISA - Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free (ab244036)

    Key features and details

    • Unconjugated capture and detector antibodies
    • Adaptable to any antibody pair-based assay format
    • Antibody concentration ~ 1 mg/ml
    • BSA and azide free buffer - ready for conjugation
    • Reacts with: Human

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    Overview

    • Product name

      Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free
      See all Alpha 1 microglobulin kits
    • Assay type

      ELISA set
    • Range

      1.56 ng/ml - 100 ng/ml
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Antibody Pair can be used to quantify Human Alpha-1-Microglobulin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


      For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab226899), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


      Download SDS here.

    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Carrier free

      Yes
    • Components 10 x 96 tests
      Human Alpha-1-Microglobulin Capture Antibody (unconjugated) 1 x 100µg
      Human Alpha-1-Microglobulin Detector Antibody (unconjugated) 1 x 100µg
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Cardiovascular
      • Blood
      • Other
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Protease inhibitors
      • Cancer
      • Tumor biomarkers
      • Other
    • Function

      Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
      Trypstatin is a trypsin inhibitor.
    • Tissue specificity

      Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
    • Sequence similarities

      In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
      Contains 2 BPTI/Kunitz inhibitor domains.
    • Post-translational
      modifications

      The precursor is proteolytically processed into separately functioning proteins.
      3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
      Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
      Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02760 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • A1M
      • Alpha 1 microglobulin/bikunin precursor
      • Alpha 1 microglycoprotein
      • Alpha-1 microglycoprotein
      • Alpha-1-microglobulin
      • AMBP
      • AMBP_HUMAN
      • Bikunin
      • Complex-forming glycoprotein heterogeneous in charge
      • EDC1
      • Growth inhibiting protein 19
      • HCP
      • HI-30
      • HI30
      • IATIL
      • ITI
      • ITI-LC
      • ITIL
      • Microglobulin (alpha 1)
      • Protein AMBP
      • Protein HC
      • Trypstatin
      • Uristatin
      • Uronic-acid-rich protein
      • UTI
      see all
    • Database links

      • Entrez Gene: 259 Human
      • Omim: 176870 Human
      • SwissProt: P02760 Human
      • Unigene: 436911 Human

      Associated products

      • Alternative Versions

        • Human Alpha-1-Microglobulin ELISA Kit (ab226899)

      Applications

      Our Abpromise guarantee covers the use of ab244036 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA Use at an assay dependent concentration.

      Images

      • Sandwich ELISA - Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free (ab244036)
        Sandwich ELISA - Human Alpha-1-Microglobulin Antibody Pair - BSA and Azide free (ab244036)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab244036? Please let us know so that we can cite the reference in this datasheet.

      ab244036 has not yet been referenced specifically in any publications.

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