Overview

  • Product name
    Human alpha 1 Microglobulin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.9%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.2 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha 1 Microglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human cell culture supernatants, serum, plasma, urine, saliva, CSF and milk.

    An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human alpha 1 Microglobulin Antibody 1 x 120µl
    alpha 1 Microglobulin Microplate (12 x 8 well strips) 1 unit
    alpha 1 Microglobulin Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificity
    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similarities
    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    modifications
    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Alpha-1 microglycoprotein
    • AMBP
    • AMBP_HUMAN
    • Bikunin
    • Complex-forming glycoprotein heterogeneous in charge
    • EDC1
    • HI-30
    • ITI-LC
    • Protein HC
    • Trypstatin
    • Uronic-acid-rich protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 10000-40000 fold. Human urine and milk diluted 100-800 fold. Human saliva diluted 8-16 fold. HepG2 supernatant diluted 1000 fold. MG-63 supernatant tested undiluted - below detection. Mouse and rat samples diluted 10 fold - below detection.

Protocols

References

This product has been referenced in:
  • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . Read more (PubMed: 28122020) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provide you with any information as to the site of recognition involved.

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Answer

Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily for small hydrophobic molecules. It contains 184 amino acids and weighs 26-kDa (1-2). Mature 1M and bikunin (urinary trypsin inhibitor) result from a common precursor (3). (1) Grubb AO et al. (1983) Biol Chem. 258:14698-14707 (2) Ekström B and Berggård I. (1977) J Biol Chem 252:8048-8057 (3) Vetr H and Gebhard W (1990) Biol Chem Hoppe Seyler. 371:1185-1196 https://www.abcam.com/alpha-1-Microglobulin-Human-ELISA-Kit-ab108884.html http://www.uniprot.org/uniprot/P02760#PRO_0000017886 ab108883 is an is an alpha 2 macroglobulin human ELISA kit, Swiss Prot P01023. It's a major serum protein. (1) Pineda-Salgado L et al (2005) Gene Expr Patterns. 6(1):3-10 (2) Jensen PE et al (2004) Biocim Biophys Acta. 5; 1690(3): 203-7 (3) Yang AH et al (1997) Nephrol Dial Transplant 12(3): 465-9 (4) Shiota G et al (1995) J Med 26(5-6): 295-308. https://www.abcam.com/alpha-2-Macroglobulin-Human-ELISA-Kit-ab108883.html http://www.uniprot.org/uniprot/P01023 ab108823 is a complement C3 human ELISA kit, Swiss Prot P01024.  Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185,000. (1) Sahu A et al. (2001) Immunol. Rev. 180: 35-48 (2) Sacks S et al. 92003) J Mol Med. 81(7): 404-10 (3) Ramos-Casals M et al. (2004) Rheumatology (Oxford). (4) Gardinali M et al. (1998) Clin.Immunol.Immunopathol .87(3): 97-303 https://www.abcam.com/Complement-C3-Human-ELISA-kit-ab108823.html http://www.uniprot.org/uniprot/P01024 ab108841 is a fibrinogen human ELISA kit, Swiss Prot P02671, P02675, and P02679.  Fibrinogen (FBG) is a homodimer of molecular mass 340 kDa, made up of two sets of α, β, γ polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte (1). FBG plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, FBG self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. FBG also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation (2). Elevated plasma FBG has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease (3, 4). Individuals with congenital absence of FBG, termed afibrinogenemia, have prolonged bleeding times. (1) Doolittle, R.F. (1984) Annu. Rev. Biochem 53:195 (2) Handley, D.A. and Hughes, T.E. (1997) Thromb. Res. 87:1 (3) Handa, K. et al. (1989) Atherosclerosis 77:209 (4) Mannucci, P.M. and Mari, D. (1993) Fibrinolysis 3:51 (5) Amiral J. (1995) Clin. Appl. Thrombosis Hemostasis 1:243 http://www.uniprot.org/uniprot/P02671 http://www.uniprot.org/uniprot/P02675 http://www.uniprot.org/uniprot/P02679 I hope this information helps.  Please contact us with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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