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    human-alpha-fetoprotein-elisa-kit-afp-ab108838.pdf

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Cardiovascular Blood Serum Proteins
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Human alpha Fetoprotein ELISA Kit (AFP) (ab108838)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (4)References (5)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.5 ng/ml
    • Range: 0.313 ng/ml - 20 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    You may also be interested in

    Protein
    Product image
    Recombinant Human alpha 1 Fetoprotein (ab114216)

    View more associated products

    Overview

    • Product name

      Human alpha Fetoprotein ELISA Kit (AFP)
      See all alpha 1 Fetoprotein kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 5.9%
      Inter-assay
      Sample n Mean SD CV%
      Overall 10.4%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      = 0.5 ng/ml
    • Range

      0.313 ng/ml - 20 ng/ml
    • Recovery

      98 %

    • Assay time

      4h 0m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum and cell culture supernatants.


      An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human alpha Fetoprotein Antibody 1 x 120µl
      alpha Fetoprotein Microplate (12 x 8 well strips) 1 unit
      alpha Fetoprotein Standard 1 vial
      Chromogen Substrate 1 x 7ml
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cancer
      • Tumor biomarkers
      • Tumor antigens
      • Developmental Biology
      • Organogenesis
      • Gut development
      • Liver development
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cardiovascular ELISA kits
    • Function

      Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • Tissue specificity

      Plasma. Synthesized by the fetal liver and yolk sac.
    • Sequence similarities

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Developmental stage

      Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
    • Post-translational
      modifications

      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02771 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Afp
      • AFPD
      • Alpha fetoglobulin
      • Alpha fetoprotein
      • Alpha fetoprotein precursor
      • Alpha-1-fetoprotein
      • Alpha-fetoglobulin
      • Alpha-fetoprotein
      • alpha-fetoprotein, Hereditary persistence of, included
      • FETA
      • FETA_HUMAN
      • Hereditary persistence of alpha fetoprotein
      • HPAFP
      see all
    • Database links

      • Entrez Gene: 174 Human
      • Omim: 104150 Human
      • SwissProt: P02771 Human
      • Unigene: 518808 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab108838.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (5)

        Publishing research using ab108838? Please let us know so that we can cite the reference in this datasheet.

        ab108838 has been referenced in 5 publications.

        • Kats D  et al. Volasertib preclinical activity in high-risk hepatoblastoma. Oncotarget 10:6403-6417 (2019). PubMed: 31741706
        • Yang J  et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). PubMed: 29754444
        • Zhang A  et al. Development and application of a fluorescence protein microarray for detecting serum alpha-fetoprotein in patients with hepatocellular carcinoma. J Int Med Res 44:1414-1423 (2016). PubMed: 27885040
        • Rikhi R  et al. Murine model of hepatic breast cancer. Biochem Biophys Rep 8:1-5 (2016). PubMed: 28955934
        • Zhang A  et al. A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index. Sci Rep 6:31132 (2016). Sandwich ELISA ; Human . PubMed: 27528397

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        1-4 of 4 Abreviews or Q&A

        Question

        Can you help me out with this?


        Read More

        Abcam community

        Verified customer

        Asked on Jul 18 2012

        Answer

        The solubility is very good. It can go to higher than 10 ug/ml. If you do in the ng/ml range, it will be in the >500 ng/ml

        Let me know if there is anything else I can help you with.

        Read More

        Abcam Scientific Support

        Answered on Jul 18 2012

        Question

        Thank you,

        I didn’t see that it was for research purposes only until later after I sent you the email. Thank you for the response!

        Read More

        Abcam community

        Verified customer

        Asked on Mar 23 2012

        Answer

        Thanks no problem. If you have any further questions relating to our products please do not hesitate to contact us again.

        Read More

        Abcam Scientific Support

        Answered on Mar 23 2012

        Question

        Hello AbCam,

        I have seen the AFP ELISA kit on your website ad would be very interested in purchasing it if it is FDA approved for research purposes. Please let me know at your convenience. Thank you very much for your time.

        Read More

        Abcam community

        Verified customer

        Asked on Mar 22 2012

        Answer

        Thank you for contacting us.

        The alpha Fetoprotein Human ELISA Kit (ab108838) has not had FDA approval but this is not necessary if the kit is to be used for research purposes only. All of our products are not intended to be used for diagnostic of therapeutic use. Please refer to our terms and conditions for further information:

        https://www.abcam.com/index.html?pageconfig=terms

        If you areintending to use the kit as specified on thedatasheet, thekit would beguaranteed for6 months by the Abpromise.More information on this guaranteecan be found from the following link:

        https://www.abcam.com/abpromiseI

        Ihope this information has been of help. If you require any further information please do not hesitate to contact us again.

        Read More

        Abcam Scientific Support

        Answered on Mar 22 2012

        Question

        Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?  

        Read More

        Abcam community

        Verified customer

        Asked on Nov 01 2011

        Answer

        Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

        Read More

        Abcam Scientific Support

        Answered on Nov 01 2011

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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