Overview

  • Product name
    Human alpha Fetoprotein ELISA Kit (AFP)
    See all alpha 1 Fetoprotein kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.5 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 0m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum and cell culture supernatants.

    An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human alpha Fetoprotein Antibody 1 x 120µl
    alpha Fetoprotein Microplate (12 x 8 well strips) 1 unit
    alpha Fetoprotein Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity
    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Afp
    • AFPD
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108838 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108838.

    Protocols

    References

    This product has been referenced in:
    • Zhang A  et al. A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index. Sci Rep 6:31132 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27528397) »

    See 1 Publication for this product

    Publishing research using ab108838? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Can you help me out with this?


    The solubility is very good. It can go to higher than 10 ug/ml. If you do in the ng/ml range, it will be in the >500 ng/ml

    Let me know if there is anything else I can help you with.

    Thank you,

    I didn’t see that it was for research purposes only until later after I sent you the email. Thank you for the response!

    Thanks no problem. If you have any further questions relating to our products please do not hesitate to contact us again.

    Hello AbCam,

    I have seen the AFP ELISA kit on your website ad would be very interested in purchasing it if it is FDA approved for research purposes. Please let me know at your convenience. Thank you very much for your time.

    Thank you for contacting us.

    The alpha Fetoprotein Human ELISA Kit (ab108838) has not had FDA approval but this is not necessary if the kit is to be used for research purposes only. All of our products are not intended to be used for diagnost...

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    Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?

    Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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