Human alpha Fetoprotein ELISA Kit (AFP) (ab108838)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameHuman alpha Fetoprotein ELISA Kit (AFP)
See all alpha 1 Fetoprotein kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity= 0.5 ng/ml
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Range0.313 ng/ml - 20 ng/ml
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Recovery
97 %
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Assay time4h 0m
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum and cell culture supernatants.
An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human alpha Fetoprotein Antibody 1 x 120µl alpha Fetoprotein Microplate (12 x 8 well strips) 1 unit alpha Fetoprotein Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
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Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
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Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
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Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- Afp
- AFPD
- Alpha fetoglobulin
see all -
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
Applications
Our Abpromise guarantee covers the use of ab108838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab108838.
Protocols
References
This product has been referenced in:
- Yang J et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). Read more (PubMed: 29754444) »
- Zhang A et al. A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index. Sci Rep 6:31132 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27528397) »
