Key features and details
- One-wash 90 minute protocol
- Sensitivity: 73 ng/ml
- Range: 1.09 ng/ml - 70 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman ALPL ELISA Kit
See all Alkaline Phosphatase kits
Intra-assay Sample n Mean SD CV% Extract 24 2.7% Inter-assay Sample n Mean SD CV% Extract 3 2.5%
Sample typeCell Lysate
Assay typeSandwich (quantitative)
Range1.09 ng/ml - 70 ng/ml
Sample specific recovery Sample type Average % Range Cell Lysate 86 81% - 95%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human ALPL ELISA Kit (ab254503) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALPL protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALPL with 73 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ALPL, also known as tissue-nonspecific alkaline phosphatase, catalyzes phosphate ester hydrolysis when in a basic environment. Deficiency in this protein leads to loss in mineralization of bone and teeth which can be fatal. ALPL is currently being used a prognostic marker for the treatment of metastatic castration-resistant prostate cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human ALPL Capture Antibody 1 x 600µl 10X Human ALPL Dectector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human ALPL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase, germ cell
- Alkaline phosphatase, testicular and thymus
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The ALPL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of ALPL were measured in duplicate and interpolated from the ALPL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALPL concentration was determined to be 59.73 ng/mL.
ab254503 has not yet been referenced specifically in any publications.