Human ALT ELISA Kit (ab234578)
- Datasheet
- References
- Protocols
Overview
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Product name
Human ALT ELISA Kit
See all Alanine Transaminase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.5% -
Sample type
Serum, EDTA Plasma, Citrate Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
47.34 pg/ml -
Range
109.38 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117 % - % EDTA Plasma 111 % - % Citrate Plasma 92 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
ALT in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ALT protein in human serum and plasma samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ALT Capture Antibody 1 x 600µl 10X Human ALT Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human ALT Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see all -
Database links
- Entrez Gene: 2875 Human
- Omim: 138200 Human
- SwissProt: P24298 Human
- Unigene: 103502 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab234578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human ALT ELISA Kit (ab234578)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ALT standard curve in Sample Diluent NS + Enhancer.The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ALT in human serum, plasma (EDTA) and plasma (citrate) samples.The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 4,002.9 pg/mL in serum, 2,685.5 pg/mL in plasma (EDTA) and 1,886.6 pg/mL in plasma (citrate).
Protocols
References
ab234578 has not yet been referenced specifically in any publications.
