Human ALT ELISA Kit (ab234578)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 47.34 pg/ml
- Range: 109.38 pg/ml - 7000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ALT ELISA Kit
See all Alanine Transaminase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.5% -
Sample type
Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
47.34 pg/ml -
Range
109.38 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117 % - % Cell culture media 95 % - % EDTA Plasma 111 % - % Cit plasma 92 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ALT ELISA Kit (ab234578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in cell culture media, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALT with 47.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only.
Tissue extract samples have not been tested with this kit.
This kit is incompatible with plasma- heparin and cell extract samples.
SPECIES REACTIVITY This kit recognizes human ALT protein. Other species reactivity not determined. Please contact our Technical Support team for more information. -
Notes
ALT (Alanine aminotransferase 1, ALT1) is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ALT Capture Antibody 1 x 600µl 10X Human ALT Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human ALT Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see all -
Database links
- Entrez Gene: 2875 Human
- Omim: 138200 Human
- SwissProt: P24298 Human
- Unigene: 103502 Human
Associated products
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Alternative Versions
Images
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Other - Human ALT ELISA Kit (ab234578)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ALT standard curve in Sample Diluent NS + Enhancer.The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human ALT standard curve in Sample Diluent NS + 1X Enhancer. The ALT standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ALT in human serum, plasma (EDTA) and plasma (citrate) samples.The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 4,002.9 pg/mL in serum, 2,685.5 pg/mL in plasma (EDTA) and 1,886.6 pg/mL in plasma (citrate).
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native ALT was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
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Linearity of dilution.Recombinant ALT was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer.
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Sandwich ELISA - Human ALT ELISA Kit (ab234578)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234578 has been referenced in 1 publication.
- Kang JA et al. Ciclopirox inhibits Hepatitis B Virus secretion by blocking capsid assembly. Nat Commun 10:2184 (2019). PubMed: 31097716