Overview

  • Product name

    Human Amphiregulin ELISA Kit
    See all Amphiregulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Amphiregulin 3 1.8%
    Inter-assay
    Sample n Mean SD CV%
    Amphiregulin 5 4.1%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.4 pg/ml
  • Range

    18 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106 84% - 117%
    Cell culture media 108 106% - 110%
    EDTA Plasma 98 91% - 104%
    Citrate Plasma 103 93% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Product overview

    Amphiregulin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Amphiregulin protein in serum, plasma (citrate and EDTA), and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Amphiregulin Capture Antibody 1 x 600µl
    10X Human Amphiregulin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI 1 x 6ml
    Human Amphiregulin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
  • Sequence similarities

    Belongs to the amphiregulin family.
    Contains 1 EGF-like domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • A REG
    • Amphiregulin
    • Amphiregulin B
    • AR
    • AREG
    • AREG_HUMAN
    • AREGB
    • Colorectum cell derived growth factor
    • Colorectum cell-derived growth factor
    • CRDGF
    • MGC13647
    • Schwannoma derived growth factor
    • SDGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The Amphiregulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Amphiregulin were measured in duplicates, interpolated from the Amphiregulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Human MCF7s were stimulated with 2% PHA for 3 days before the supernatant was collected and assayed. The concentrations of Amphiregulin were measured in duplicate and interpolated from the Amphiregulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amphiregulin concentration was determined to be 23.7 ng/mL in stimulated and 16 ng/mL in unstimulated MCF7 cell culture supernatant.

Protocols

References

ab222504 has not yet been referenced specifically in any publications.

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