Human Amphiregulin ELISA Kit (AREG) (ab99975)


  • Product name
    Human Amphiregulin ELISA Kit (AREG)
    See all Amphiregulin kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    16.46 pg/ml - 4000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.4 89% - 111%
    Plasma 72.7 64% - 87%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Amphiregulin (AREG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in plasma, and cell culture supernatants. (Human Amphiregulin concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human Amphiregulin in normal serum with ab99975, only in serum spiked with Human Amphiregulin.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Amphiregulin Microplate (12 x 8 well strips) 1 unit
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Amphiregulin (lyophilized) 2 vials
    Recombinant Human Amphiregulin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    The mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • A REG
    • AR
    • AREG
    • Colorectum cell derived growth factor
    • CRDGF
    • MGC13647
    • OTTHUMP00000219184
    • Schwannoma derived growth factor
    • SDGF
    see all
  • Database links


Our Abpromise guarantee covers the use of ab99975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab99975.

  • Representative Standard Curve using ab99975.



ab99975 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

A lot of our kits are very accommodating to many different samples types so lysates will likely work. If you decide to tests this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any...

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