Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.1 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Amyloid beta 1-40 ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.1 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Serum 101.3 77% - 125% Plasma 97.39 68% - 136% Cell culture media 104.3 71% - 145%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Predicted to work with: Mouse
Abcam’s Amyloid beta 1-40 Human ELISA Kit (ab193692) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amyloid beta 1-40 in serum, plasma, and cell culture supernatants. (UniprotID: P05067)
This assay employs an antibody specific for Human Amyloid beta 1-40 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples. The wells are washed and biotinylated anti-Human Amyloid beta 1-40 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 1100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Human Amyloid beta detection antibody 2 vials Human Amyloid beta Standards (lyophilized) 2 vials Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: P05067 Human
ab193692 has been referenced in 1 publication.
- Kim K et al. Chemical sensing platforms for detecting trace-level Alzheimer's core biomarkers. Chem Soc Rev 49:5446-5472 (2020). PubMed: 32627779