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    human-angiogenesis-antibody-array-60-targets-quantitative-ab197418.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Human Angiogenesis Antibody Array (60 Targets) - Quantitative (ab197418)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human Angiogenesis Antibody Array (60 Targets) - Quantitative Assay Summary (ab197418)

    Overview

    • Product name

      Human Angiogenesis Antibody Array (60 Targets) - Quantitative
      See all Angiogenesis Antibody Array antibody arrays
    • Sample type

      Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
    • Assay type

      Quantitative
    • Assay time

      6h 00m
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab197418 can be used for quantitative measurement of 60 Human angiogenesis markers. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
      Targets names: Activin A, AgRP, Angiogenin, ANG-2, ANGPTL4, bFGF, ENA-78, GRO, HB-EGF, HGF, IFNγ, IGF-I, IL-1α, IL-2, IL-6, IL-8, IL-17, IP-10, Leptin, LIF, MCP-1, PDGF-BB, PIGF, RANTES, TGFβ1, TIMP-1, TIMP-2, TNFα, TNFβ, TPO, ANG-1, Angiostatin, CXCL16, EGF, FGF-4, Follistatin, G-CSF, GM-CSF, I-309, IL-1β, IL-4, IL-10, IL-12p40, IL-12p70, I-TAC, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TGFα, TGFβ3, Tie-1, Tie-2, uPAR, VEGF, VEGF R2, VEGF R3, VEGF-D. 


      The analytes measured by this kit are split across 2 different arrays on different slides, both of which are provided in the kit.
      Learn more about quantitative antibody arrays

    • Notes

      Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.


      Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.


      For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.


      Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.


      The standard is affinity purified.


      Learn more about quantitative antibody arrays


      Learn more about membrane antibody arrays

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 8 tests 22 tests 50 tests
      20X Wash Buffer I 3 x 30ml 6 x 30ml 12 x 30ml
      20X Wash Buffer II 1 x 30ml 2 x 30ml 4 x 30ml
      Cy3 equivalent dye-conjugated Streptavidin 2 x 5µl 4 x 5µl 8 x 5µl
      Human Angiogenesis Array A (30 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units
      Human Angiogenesis Array A (30 T) Glass Slide 1 slide 2 slides 4 slides
      Human Angiogenesis Array A (30 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 4 vials
      Human Angiogenesis Array B (30 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units
      Human Angiogenesis Array B (30 T) Glass Slide 1 slide 2 slides 4 slides
      Human Angiogenesis Array B (30 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 4 vials
      Sample Diluent 1 x 15ml 2 x 15ml 4 x 15ml
      Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 2 units 4 units
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Antibody Arrays
      • Membrane antibody arrays
      • Angiogenesis antibody arrays

    Images

    • Human Angiogenesis Antibody Array (60 Targets) - Quantitative Assay Summary (ab197418)
      Human Angiogenesis Antibody Array (60 Targets) - Quantitative Assay Summary (ab197418)

      Assay Summary for Human Angiogenesis Antibody Array A (30 Targets) - Quantitative (ab197418).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab197418? Please let us know so that we can cite the reference in this datasheet.

    ab197418 has been referenced in 1 publication.

    • Zhao L  et al. TERT assists GDF11 to rejuvenate senescent VEGFR2+/CD133+ cells in elderly patients with myocardial infarction. Lab Invest 99:1661-1688 (2019). PubMed: 31292540

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