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    human-angiogenesis-antibody-array-membrane-20-targets-ab134000.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)

  • Datasheet
  • SDS
  • Protocol Booklet
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Conditioned media
  • Human serum and plasma
  • Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)

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Human Cytokine Antibody Array I (Membrane, 37 Targets) (ab211059)

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Overview

  • Product name

    Human Angiogenesis Antibody Array - Membrane (20 Targets)
    See all Angiogenesis Antibody Array antibody arrays
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab13400 is for simultaneous detection of 20 Human Angiogenic factors. Suitable for all sample types.


    Targets: Angiogenin, EGF, ENA-78, bFGF, GRO, IFN-gamma, IGF-I, IL-6, IL-8, Leptin, MCP-1, PDGF-BB, PIGF, RANTES, TGF-beta1, TIMP-1, TIMP-2, Thrombopoietin, VEGF-A, VEGF-D


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133997, ab133998 and ab169819 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Biotinylated Antibody Cocktail (C1) 2 vials 4 vials
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
    Human Angiogenesis Antibody Array Membranes (C1) 4 units 8 units
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Antibody Arrays
    • Membrane antibody arrays
    • Angiogenesis antibody arrays
    • Antibody Arrays
    • Membrane antibody arrays
    • EGFR phosphorylation antibody arrays

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab134000 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.
    Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.

    Images

    • Conditioned media
      Conditioned media

      THP-1 cells (Human peripheral blood monocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.05mM 2-mercaptoethanol, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured for 2 days in the presence of LPS. Conditioned media was harvested after 48 hours post-stimulation, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

      HUVEC cells (Human umbilical vein endothelial cells) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.1 mg/mL heparin, 0.05 mg/mL ECGS, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

    • Human serum and plasma
      Human serum and plasma

      Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.

    • Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)
      Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)

      Typical results obtained with Abcam Human Cytokine Antibody Array - Membrane. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab134000? Please let us know so that we can cite the reference in this datasheet.

    ab134000 has not yet been referenced specifically in any publications.

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