Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)
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- References
- Protocols
Overview
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Product nameHuman Angiogenesis Antibody Array - Membrane (20 Targets)
See all Angiogenesis Antibody Array antibody arrays -
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
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Assay typeSemi-quantitative
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Species reactivityReacts with: Human
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Product overview
ab13400 is for simultaneous detection of 20 Human Angiogenic factors. Suitable for all sample types.
Targets: Angiogenin, EGF, ENA-78, bFGF, GRO, IFN-gamma, IGF-I, IL-6, IL-8, Leptin, MCP-1, PDGF-BB, PIGF, RANTES, TGF-beta1, TIMP-1, TIMP-2, Thrombopoietin, VEGF-A, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Notes
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Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotinylated Antibody Cocktail (C8) 2 vials 4 vials Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Human Angiogenesis Antibody Array Membranes (C1) 4 units 8 units -
Research areas
Applications
Our Abpromise guarantee covers the use of ab134000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Conditioned mediaTHP-1 cells (Human peripheral blood monocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.05mM 2-mercaptoethanol, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured for 2 days in the presence of LPS. Conditioned media was harvested after 48 hours post-stimulation, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
HUVEC cells (Human umbilical vein endothelial cells) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 0.1 mg/mL heparin, 0.05 mg/mL ECGS, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
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Human serum and plasmaHuman serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134000. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Angiogenesis Antibody Array - Membrane (20 Targets) (ab134000)Typical results obtained with Abcam Human Cytokine Antibody Array - Membrane. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
Protocols
References
ab134000 has not yet been referenced specifically in any publications.
