Product nameHuman Angiogenesis Antibody Array - Membrane (23 targets)
See all Angiogenesis Antibody Array antibody arrays
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
Species reactivityReacts with: Human
ab169808 is for simultaneous detection of 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Targets: Angiopoietin 1, Angiopoietin 2, Angiostatin, Endostatin, G-CSF, GM-CSF, I-309, IL-10, IL-1 alpha, IL-1 beta, IL-2, IL-4, I-TAC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 4 Membranes 1 x 8 Membranes Biotinylated Antibody Cocktail (C2) 2 vials 4 vials 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Human Angiogenesis Antibody Array Membranes (C2) 1 x 4 units 1 x 8 units 1X Blocking Buffer 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml
Our Abpromise guarantee covers the use of ab169808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab169808 has not yet been referenced specifically in any publications.