Overview

  • Product name

    Human Angiogenin ELISA Kit
    See all Angiogenin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.6%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Cell culture media, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.06 pg/ml
  • Range

    7.81 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 108% - 113%
    Serum 103 99% - 106%
    Cell culture extracts 101 99% - 103%
    Cell culture media 104 100% - 107%
    Heparin Plasma 103 96% - 109%
    EDTA Plasma 100 96% - 102%
    Citrate Plasma 101 97% - 105%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    Angiogenin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Angiogenin protein in serum, plasma, cell culture supernatant, and cell extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS - 3.12 pg/mL
    Samples in 1X Cell Extraction Buffer PTR - 1.06 pg/mL

  • Notes

    Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence homology with pancreatic RNase. Angiogenin can act to inhibit protein synthesis by functioning as a cytotoxic tRNA-specific RNase.

    Cells expressing Angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, colonic epithelium, peripheral blood lymphocytes, lung cell lines, and primary gastrointestinal adenocarcinomas. Basal levels of Angiogenin are also present in normal human serum and plasma. Angiogenin has been reported to serve as protection for motor neurons and it follows that Angiogenin is associated with neurological diseases, specifically, mutations to this gene are linked to Amyotrophic Lateral Sclerosis (ALS). Human and mouse Angiogenin share 74% homology in amino acid sequence.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab219629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:1,000, and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.53 ng/mL in neat serum, 310.16 ng/mL in neat plasma (citrate), 249.90 ng/mL in neat plasma (EDTA), and 329.36 ng/mL in neat plasma (heparin).

  • The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: RPMI culture media 50% and HepG2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 527.60 pg/mL in neat, spiked RPMI culture media. The mean Angiogenin concentration was determined to be 945.93 pg/mL in neat, native HepG2 supernatant.

  • The concentrations of Angiogenin were measured in duplicate and interpolated from the Angiogenin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 304.03 pg/mL in HepG2 cell extract sample.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 495.29 ng/mL with a range of 214.57 – 824.72 ng/mL.

Protocols

References

ab219629 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219629.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up