Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 1.64 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Angiogenin ELISA Kit (ANG)
See all Angiogenin kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1.5 pg/ml
Range1.64 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.37 85% - 104% Serum 95.95 82% - 105% Plasma 93.49 83% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.
This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Angiogenin Microplate (12 x 8 well strip) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-Human Angiogenin (lyophilized) 2 vials Recombinant Human Angiogenin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceAngiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence identity with pancreatic RNase. Like other members of the ribonuclease superfamily, angiogenin is a cytotoxic agent that can eliminate cellular protein synthesis. Angiogenin causes inhibition of protein synthesis by functioning as a cytotoxic tRNA-specific RNase. Angiogenesis is the formation of blood vessels or capillaries from existing blood vessels due to specific signals. Angiogenin induces the growth of new blood vessels. It stimulates capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin exhibits both angiogenic and non-angiogenic activities and is involved in endothelial cell migration, proliferation, and differentiation. It is produced by a variety of tumor and normal cell types. Cells that express angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. Angiogenin is also present in normal human plasma.
Cellular localizationNuclear and Secreted
- ANG 1
ab99970 has been referenced in 5 publications.
- Ting KR et al. Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients. BBA Clin 8:28-34 (2017). PubMed: 28725572
- Peres R et al. Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation. Oncotarget 7:43109-43123 (2016). PubMed: 27317771
- Yeh CH et al. Poly(Dopamine)-Assisted Immobilization of Xu Duan on 3D Printed Poly(Lactic Acid) Scaffolds to Up-Regulate Osteogenic and Angiogenic Markers of Bone Marrow Stem Cells. Materials (Basel) 8:4299-4315 (2015). PubMed: 28793441
- Giacoia EG et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). PubMed: 24464915
- Miyake M et al. Angiogenin promotes tumoral growth and angiogenesis by regulating matrix metallopeptidase-2 expression via the ERK1/2 pathway. Oncogene N/A:N/A (2014). PubMed: 24561529