Overview

  • Product name

    Human Angiogenin ELISA Kit (ANG)
    See all Angiogenin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1.5 pg/ml
  • Range

    1.64 pg/ml - 400 pg/ml
  • Recovery

    94.7 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.37 85% - 104%
    Serum 95.95 82% - 105%
    Plasma 93.49 83% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab99970.

  • Representative standard curve using ab99970.

Protocols

References

This product has been referenced in:

  • Ting KR  et al. Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients. BBA Clin 8:28-34 (2017). Read more (PubMed: 28725572) »
  • Peres R  et al. Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation. Oncotarget 7:43109-43123 (2016). Read more (PubMed: 27317771) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. I have confirmed that Assay Diluent B would be the best diluent to use if testing tears.  Assay Diluent A is specifically optimized to simulate the serum/plasma matrix while Assay Diluent B is better suited for a variety of sample types. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up