Overview

  • Product name
    Human Angiopoietin 1 ELISA Kit (ANG1)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    74 pg/ml - 18000 pg/ml
  • Recovery

    < 95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 83% - 105%
    Serum 94.47 81% - 103%
    Plasma 93.93 80% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Angiopoietin 1 (ANG1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Angiopoietin 1 Microplate (12 x 8 well strips) 1 unit
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Angiopoietin 1 (lyophilized) 2 vials
    Recombinant Angiopoietin 1 Standard Human (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin 1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development. Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.
  • Alternative names
    • 1110046O21Rik
    • AGP 1
    • AGP1
    • AGPT
    • ANG 1
    • ANG1
    • Angiopoietin1
    • ANGPT 1
    • ANGPT1
    • KIAA0003
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab99972 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • TYPICAL STANDARD CURVE - Assay DIluent A
      TYPICAL STANDARD CURVE - Assay DIluent A
      TYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
    • TYPICAL STANDARD CURVE - Assay Diluent B
      TYPICAL STANDARD CURVE - Assay Diluent B
      TYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

    Protocols

    References

    This product has been referenced in:
    • Ollivier V  et al. Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI. PLoS One 9:e104712 (2014). Read more (PubMed: 25116206) »

    See 1 Publication for this product

    Publishing research using ab99972? Please let us know so that we can cite the reference in this datasheet.

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    Inquiry: Dear Abcam. Can you please inform me who or which company is the distributor of Abcam products in Denmark. Thank you. Sincerely,

    Thank you for your enquiry.

    I can confirm that from Denmark you can order directly from us. We have no official distributors in Denmark.

    To place your order, you can do this via the website, or send your order to our customer serv...

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