Human Angiopoietin 1 ELISA Kit (ANG1) (ab99972)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 74 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Angiopoietin 1 ELISA Kit (ANG1) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 30 pg/ml -
Range
74 pg/ml - 18000 pg/ml -
Recovery
< 95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.25 83% - 105% Serum 94.47 81% - 103% Plasma 93.93 80% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Angiopoietin 1 (ANG1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Angiopoietin 1 Microplate (12 x 8 well strips) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-Human Angiopoietin 1 2 vials Recombinant Angiopoietin 1 Standard Human (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin 1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development. Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart. -
Alternative names
- 1110046O21Rik
- AGP 1
- AGP1
see all -
Database links
- SwissProt: Q15389 Human
Images
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TYPICAL STANDARD CURVE - Assay DIluent ATYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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TYPICAL STANDARD CURVE - Assay Diluent BTYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab99972 has been referenced in 4 publications.
- Yi X et al. Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions. Stem Cell Res Ther 11:183 (2020). PubMed: 32430063
- Borrelli MR et al. CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat. Stem Cells Transl Med 9:1389-1400 (2020). PubMed: 32543083
- Fei J et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757
- Ollivier V et al. Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI. PLoS One 9:e104712 (2014). PubMed: 25116206