For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    human-angiopoietin-1-elisa-kit-ang1-ab99972.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Growth Factors Angiopoietin
Share by email

Human Angiopoietin 1 ELISA Kit (ANG1) (ab99972)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

TYPICAL STANDARD CURVE - Assay DIluent A
  • TYPICAL STANDARD CURVE - Assay Diluent B

Key features and details

  • Sensitivity: 30 pg/ml
  • Range: 74 pg/ml - 18000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

ELISA
Product image
Human VEGF ELISA Kit (ab100662)
Kit
Product image
Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)
Pair
Product image
Human IL-1beta Antibody Pair - BSA and Azide free (ab241807)

View more associated products

Overview

  • Product name

    Human Angiopoietin 1 ELISA Kit (ANG1)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    74 pg/ml - 18000 pg/ml
  • Recovery

    < 95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 83% - 105%
    Serum 94.47 81% - 103%
    Plasma 93.93 80% - 102%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Angiopoietin 1 (ANG1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Angiopoietin 1 Microplate (12 x 8 well strips) 1 unit
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Angiopoietin 1 2 vials
    Recombinant Angiopoietin 1 Standard Human (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Angiopoietin
    • Cardiovascular
    • Angiogenesis
    • Lymph Angiogenesis
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Angiogenic factors ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
  • Relevance

    Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin 1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development. Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.
  • Alternative names

    • 1110046O21Rik
    • AGP 1
    • AGP1
    • AGPT
    • ANG 1
    • ANG1
    • Angiopoietin1
    • ANGPT 1
    • ANGPT1
    • KIAA0003
    see all
  • Database links

    • SwissProt: Q15389 Human

    Images

    • TYPICAL STANDARD CURVE - Assay DIluent A
      TYPICAL STANDARD CURVE - Assay DIluent A
      TYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
    • TYPICAL STANDARD CURVE - Assay Diluent B
      TYPICAL STANDARD CURVE - Assay Diluent B
      TYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab99972? Please let us know so that we can cite the reference in this datasheet.

    ab99972 has been referenced in 4 publications.

    • Yi X  et al. Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions. Stem Cell Res Ther 11:183 (2020). PubMed: 32430063
    • Borrelli MR  et al. CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat. Stem Cells Transl Med 9:1389-1400 (2020). PubMed: 32543083
    • Fei J  et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757
    • Ollivier V  et al. Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI. PLoS One 9:e104712 (2014). PubMed: 25116206

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Inquiry: Dear Abcam. Can you please inform me who or which company is the distributor of Abcam products in Denmark. Thank you. Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2012

    Answer

    Thank you for your enquiry.

    I can confirm that from Denmark you can order directly from us. We have no official distributors in Denmark.

    To place your order, you can do this via the website, or send your order to our customer service department at mailto:orders@abcam.com

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Sep 21 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.