Key features and details
- Sensitivity: 18.75 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameHuman Angiotensin II ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeSerum, Plasma, Other biological fluids, Tissue Homogenate
Sensitivity< 18.75 pg/ml
Range31.25 pg/ml - 2000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human Angiotensin II ELISA Kit (ab285306, E4527) is a competitive ELISA assay for the quantitative measurement of Ang II in Human serum, plasma, tissue homogenates and other biological fluids. The microtiter plate provided in this kit has been pre-coated with the target antigen. During the reaction, the target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. The unbound sample or excess conjugate are washed from the plate. HRP-Streptavidin (SABC) is added and incubated. Then TMB substrate solution is added to each well. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is measure at 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 96 tests 96 tests 25X Wash buffer 1 x 30ml 1 x 30ml Antibody dilution buffer 1 x 10ml 1 x 10ml Biotin/Detection antibody (Concentrated) 1 x 60µl 1 x 60µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl 1 x 120µl Lyophilized Standard 2000 pg 2 vials 2 vials Micro ELISA Plate 1 unit 1 unit Plate sealers 5 units 5 units SABC dilution buffer 1 x 10ml 1 x 10ml Sample / Standard Dilution Buffer 1 x 20ml 1 x 20ml Stop Solution 1 x 10ml 1 x 10ml TMB Substrate 1 x 10ml 1 x 10ml
FunctionEssential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3: stimulates aldosterone release.
Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseEssential hypertension
Renal tubular dysgenesis
Sequence similaritiesBelongs to the serpin family.
modificationsBeta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin).
The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
- Information by UniProt
- Ang I
- Ang II
ab285306 has not yet been referenced specifically in any publications.