Overview

  • Product name

    Human Annexin A1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 1 20 5.1%
    Plasma 2 20 5.1%
    Plasma 3 20 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 1 20 11%
    Plasma 2 20 10.8%
    Plasma 3 20 10.5%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.19 ng/ml
  • Range

    0.313 ng/ml - 20 ng/ml
  • Recovery

    97 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Rabbit, Cow
  • Product overview

    ab222868 Human Annexin A1 ELISA Kit is designed for the quantitative measurement of Annexin A1 in plasma, serum, cell lysate and cell culture samples.


    The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A1 in approximately 4 hours. A polyclonal antibody specific for human Annexin A1 has been pre-coated onto a 96-well microplate with removable strips. Annexin A1 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Annexin A1, also known as lipocortin I, annexin I, chromobindin-9, and calpactin II, belongs to the annexin family of calcium-dependent phospholipid-binding proteins. The annexin proteins are structurally characterized by a core domain containing a conserved 70 amino acid sequence repeated four or eight times. This core is preceded by a unique N-terminal domain that varies in sequence and length and is thought to be responsible for specifying different annexin functions. Annexin A1 has an apparent relative molecular mass of 40 kDa with 346 amino acids and is preferentially located on the cytosolic face of the plasma membrane. It mimics the effect of steroids, mediates anti-inflammatory activity and is a potent inhibitor of phospholipase A2 activity. Annexin A1 regulates neutrophil extravasation by interacting with the formyl peptide receptor and triggers different signaling pathways.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    30x Biotinylated Human Annexin A1 1 x 180µl
    Anti-Human Annexin A1 coated Microplate (12 x 8 wells) 1 unit
    Chromogen Substrate 1 x 8ml
    Human Annexin A1 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization

    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Alternative names

    • Annexin 1
    • Annexin A1
    • Annexin I
    • Annexin I (lipocortin I)
    • Annexin-1
    • AnnexinA1
    • AnnexinI
    • ANX 1
    • ANX A1
    • ANX1
    • ANXA 1
    • ANXA1
    • ANXA1 protein
    • ANXA1_HUMAN
    • Calpactin 2
    • Calpactin II
    • Calpactin-2
    • CalpactinII
    • Chromobindin 9
    • Chromobindin-9
    • Chromobindin9
    • HGNC:533
    • Lipocortin 1
    • Lipocortin I
    • Lipocortin1
    • LipocortinI
    • LPC 1
    • LPC1
    • p35
    • Phospholipase A2 inhibitory protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human Annexin A1 standard curve.

Protocols

References

ab222868 has not yet been referenced specifically in any publications.

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