Human Annexin V ELISA Kit (ab223863)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Annexin V ELISA Kit
See all Annexin V/ANXA5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 3.8% Inter-assay Sample n Mean SD CV% Supernatant 3 10.8% -
Sample type
Cell culture supernatant, Urine, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
23.4 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 98 96% - 101% Cell culture extracts 90 86% - 96% Tissue Extracts 103 100% - 105% Cell culture media 107 99% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Annexin V in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Annexin V protein in human urine, cell culture supernatant, and cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS + Enhancer = 24.7 pg/ml.
Samples diluted in 1X Cell Extraction Buffer PTR = 23.4 pg/ml.
Mouse and rat samples are not compatible with this kit
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human Annexin V Capture Antibody 1 x 600µl 10X Human Annexin V Detector Antibody 1 x 600µl Human Annexin V Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
Database links
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
Images
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Other - Human Annexin V ELISA Kit (ab223863)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Annexin V standard curve in Sample Diluent NS + Enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Annexin V in human HepG2 cell extract.Interpolated concentrations of native Annexin V in human HepG2 cell extract based on a 1 μg/mL extract load and heart and brain tissue extract samples based on a 2 μg/mL extract load. The concentrations of Annexin V were measured in duplicate and interpolated from the Annexin V standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1299 pg/mL in HepG2 cell extract, 2159 pg/mL in human heart tissue extract, and 2585 pg/mL in human brain tissue extract.
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Example of human Annexin V standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Annexin V in human urine and HepG2 cell culture supernatant (5 days) samples.The concentrations of Annexin V were measured in duplicates, interpolated from the Annexin V standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 20% and HepG2 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1601 pg/mL in neat urine and 14,220 pg/mL in neat HepG2 Cell Culture Supernatant.
Protocols
References
ab223863 has not yet been referenced specifically in any publications.
