Overview

  • Product name

    Human Antithrombin III ELISA Kit
    See all Antithrombin III kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 20 3.1%
    2 20 3.2%
    3 20 3.6%
    Inter-assay
    Sample n Mean SD CV%
    1 20 8.9%
    2 20 9%
    3 20 9.1%
  • Sample type

    Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.03 µg/ml
  • Range

    0.063 µg/ml - 4 µg/ml
  • Recovery

    96.5 %

  • Assay time

    2h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Antithrombin III ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab220073) is designed for detection of Human Antithrombin III (AT3) in plasma and serum samples.


    This assay employs a quantitative competitive enzyme immunoassay technique that measures Antithrombin III in less than 3 hours. A polyclonal antibody specific for Antithrombin III has been pre-coated onto a 96-well microplate with removable strips. Antithrombin III in standards and samples compete with a biotinylated Antithrombin III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    The serine protease inhibitor antithrombin III, the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties. AT3 levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab220073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Human ATIII Standard curve (ab220073)

Protocols

References

ab220073 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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