• Product name

    Human APH1a peptide
  • Animal free

  • Nature

    • Species



Our Abpromise guarantee covers the use of ab12394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Form

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • 6530402N02Rik
    • AL138795.3
    • Anterior Pharynx Defective 1
    • Anterior pharynx defective 1 homolog A
    • APH 1A
    • Aph 1alpha
    • APH-1a
    • Aph-1alpha
    • Aph1a
    • APH1A gamma secretase subunit
    • CGI 78
    • CGI78
    • Gamma secretase subunit APH 1A
    • Gamma Secretase Subunit APH1a
    • Gamma-secretase subunit APH-1A
    • Likely ortholog of C. elegans anterior pharynx defective 1A
    • Presenilin Stabilization Factor
    • Presenilin-stabilization factor
    • PSF
    • UNQ579/PRO1141
    see all
  • Function

    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
  • Tissue specificity

    Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
  • Sequence similarities

    Belongs to the APH-1 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Information by UniProt


  • Rabbit polyclonal to APH1a (ab12104) used at 1/500 on:

    Lane 1: Human Brain Lysate
    Lane 2: Mouse Brain Lysate
    Lane 3: Human Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)
    Lane 4:  Mouse Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394)

    Secondary antibody : Goat polyclonal to Rabbit IgG (HRP) -  pre-adsorbed at 1/5000  (ab7090).

    Expected molecular weight was 30kDa. The band observed between 20-25kDa is likely to be an isoform of Aph1a.

    We are unsure as to the identity of the minor band seen at 75kDa.

    Blot exposure time=30 seconds

    Rabbit polyclonal to APH1a (ab12104) used at 1/500 on: Lane 1: Human Brain Lysate Lane 2: Mouse Brain Lysate Lane 3: Human Brain Lysate + 1µg/ml APH1a blocking peptide (ab12394) Lane 4: Mouse Brain Lysate + 1µg/ml APH1a blocking pe

  • All lanes : Anti-APH1a antibody (ab12104) at 1 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse brain lysate with Human APH1a peptide (ab12394) at 1 µg/ml
    Lane 4 : Rat brain lysate with Human APH1a peptide (ab12394) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 21 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute


ab12394 has not yet been referenced specifically in any publications.

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