Overview

  • Product name
    Human APM2 peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab95393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Blocking - Blocking peptide for Anti-APM2 antibody (ab79579)

  • Form
    Liquid
  • Additional notes
    Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 deg C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

General Info

  • Alternative names
    • Adipose most abundant gene transcript 2 protein
    • Adipose specific 2
    • APM 2
    • C10orf116
    • Chromosome 10 open reading frame 116
    • OTTHUMP00000020019
    • RP11-96C23.4
    see all
  • Relevance
    APM2 gene is exclusively expressed in adipose tissue. Its function is currently unknown. APM2 has been shown to be associated with cisplatin resistance in cell lines. It is not found in cisplatin sensitive cell lines, implicating APM2 in the development of cisplatin resistance in tumor cells.

Images

  • Indirect ELISA - APM2 peptide (ab95393)
    Indirect ELISA - APM2 peptide (ab95393)This image is courtesy of an anonymous Abreview
    ab95393 (serially diluted from 800 ng/ml and incubated for 1 hour at 24°C) was used for establishing the standard curve.

    ab79579 was used to detect APM2 in Human serum by indirect ELISA. Samples were blocked with 5% BSA for 1 hour at 24°C and were incubated with the primary antibody (1/1000) for 3 hours at 26°C. An HRP-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

ab95393 has not yet been referenced specifically in any publications.

Publishing research using ab95393? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

ELISA Abreview for APM2 peptide

 Average
Abreviews
Application
ELISA

Review text: 1. ab79579, APM2 antibody was used.
2. APM2 peptide (ab95393) was serially diluted from 800 ng/ml .
Sample: Human Serum (Serum)
Specification: Serum
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Type: Indirect

Other product details
Concentration: 4 (unknown units)
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 24°C

Primary antibody (in addition to 'APM2 peptide')
Primary antibody: Abcam primary antibody: Anti-APM2 antibody (ab79579)
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Anti-Rabbit IgG HRP Conjugated
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/1000
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Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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