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    human-apm2-peptide-ab95393.pdf

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Human APM2 peptide (ab95393)

  • Datasheet
  • SDS
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Indirect ELISA - Human APM2 peptide (ab95393)

    Key features and details

    • Suitable for: ELISA, Blocking

    Description

    • Product name

      Human APM2 peptide
    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human

    Associated products

    • Corresponding Antibody

      • Anti-APM2 antibody (ab79579)

    Specifications

    Our Abpromise guarantee covers the use of ab95393 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Blocking - Blocking peptide for Anti-APM2 antibody (ab79579)

    • Form

      Liquid
    • Additional notes

      Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 deg C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Constituent: PBS

    General Info

    • Alternative names

      • Adipose most abundant gene transcript 2 protein
      • Adipose specific 2
      • APM 2
      • C10orf116
      • Chromosome 10 open reading frame 116
      • OTTHUMP00000020019
      • RP11-96C23.4
      see all
    • Relevance

      APM2 gene is exclusively expressed in adipose tissue. Its function is currently unknown. APM2 has been shown to be associated with cisplatin resistance in cell lines. It is not found in cisplatin sensitive cell lines, implicating APM2 in the development of cisplatin resistance in tumor cells.

    Images

    • Indirect ELISA - Human APM2 peptide (ab95393)
      Indirect ELISA - Human APM2 peptide (ab95393)This image is courtesy of an anonymous Abreview
      ab95393 (serially diluted from 800 ng/ml and incubated for 1 hour at 24°C) was used for establishing the standard curve.

      ab79579 was used to detect APM2 in Human serum by indirect ELISA. Samples were blocked with 5% BSA for 1 hour at 24°C and were incubated with the primary antibody (1/1000) for 3 hours at 26°C. An HRP-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab95393? Please let us know so that we can cite the reference in this datasheet.

    ab95393 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    ELISA Abreview for APM2 peptide

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA

    Review text: 1. ab79579, APM2 antibody was used.
    2. APM2 peptide (ab95393) was serially diluted from 800 ng/ml .
    Sample: Human Serum (Serum)
    Specification: Serum
    Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Type: Indirect

    Other product details
    Concentration: 4 (unknown units)
    Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 24°C

    Primary antibody (in addition to 'APM2 peptide')
    Primary antibody: Abcam primary antibody: Anti-APM2 antibody (ab79579)
    Dilution: 1/1000

    Secondary antibody
    Name: Non-Abcam antibody was used: Anti-Rabbit IgG HRP Conjugated
    Host species: Donkey
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/1000

    Abcam user community

    Verified customer

    Submitted Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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