Overview

  • Product name

  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Breast milk 8 2%
    Inter-assay
    Sample n Mean SD CV%
    Breast milk 3 0.8%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    235 pg/ml
  • Range

    0.7 ng/ml - 8 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 91% - 110%
    Milk 96 87% - 101%
    Urine 95 93% - 97%
    Serum 119 112% - 128%
    Cell culture media 91 85% - 98%
    Heparin Plasma 108 102% - 114%
    EDTA Plasma 117 103% - 130%
    Citrate Plasma 105 95% - 116%
    Cerebral Spinal Fluid 101 86% - 101%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    APOC3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of APOC3 protein in human serum, plasma, milk, urine, cerebrospinal fluid, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    APOC3 (Apolipoprotein C-III) is a component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. APOC3 functions in triglyceride homeostasis. Intracellularly, APOC3 promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion. Extracellularly, APOC3 attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). It impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab238268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The APOC3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The concentrations of APOC3 were measured in duplicates, interpolated from the APOC3 standard curves and corrected for sample dilution. Undiluted samples are as follows: breast milk 3.33%, urine 50%, CSF 4.44%, and HepG2 supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 188.8 ng/mL in neat breast milk, 2.895 ng/mL in neat urine, 105.5 ng/mL in neat cerebrospinal fluid, and 77.04 ng/mL in neat HepG2 cell culture supernatant.
  • The concentrations of APOC3 were measured in duplicates, interpolated from the APOC3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20,000, plasma (citrate) 1:20,000, plasma (heparin) 1:20,000, and plasma (EDTA) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 116.4 µg/mL in neat serum, 124.3 µg/mL in neat plasma (citrate), 118.8 µg/mL in neat plasma (heparin), and 168.4 µg/mL in neat plasma (EDTA).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 111.9 µg/mL with a range of 52.19 – 314.5 µg/mL.

Protocols

References

ab238268 has not yet been referenced specifically in any publications.

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