Overview

  • Product name

    Human Apolipoprotein A-IV ELISA Kit
    See all APOA4/Apo-AIV kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.4%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.4 pg/ml
  • Range

    250 pg/ml - 16000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 78 76% - 79%
    Urine 97 95% - 98%
    Serum 98 90% - 101%
    Tissue Extracts 103 102% - 105%
    Cell culture media 103 102% - 103%
    Heparin Plasma 98 95% - 101%
    EDTA Plasma 104 102% - 107%
    Citrate Plasma 91 90% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    Apolipoprotein A-IV in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Apolipoprotein A-IV protein in serum, plasma, saliva, urine, cell culture supernatants, and tissue extract samples.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS: 27.9 pg/mL


    Samples in 1X Cell Extraction Buffer PTR: 5.4 pg/mL

  • Notes

    Human Apolipoprotein A-IV is a 45kDa glycoprotein with activities involving lipid transport. Specifically, Apolipoprotein A-IV is a component of high density lipoprotein (HDL) and chylomicrons. Its primarily synthesized is the intestine in association with lymph chylomicron particles. Although its precise function is not known, Apolipoprotein A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. Human Apolipoprotein A-IV consists of a 20 amino acid (aa) signal peptide and 376 aa mature chain. Human Apolipoprotein A-IV is 62% identical to both mouse and rat Apolipoprotein A-IV.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab214567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:32,000, plasma (citrate) 1:16,000, plasma (EDTA) 1:16,000, and plasma (heparin) 1:16000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 71.65 µg/mL in neat serum, 48.04 µg/mL in neat plasma (citrate), 42.94 µg/mL in neat plasma (EDTA) and 51.18 µg/mL in neat plasma (heparin).

  • The concentrations of Apolipoprotein A-IV were measured in duplicate and interpolated from the Apolipoprotein A-IV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 4,829 pg/mL in placenta extract and 17,016 pg/mL in liver homogenate extract.

  • The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 177,941 pg/mL in neat saliva (spiked) and 80,680 pg/mL in neat urine (spiked).

  • Saliva was analyzed at 6.25% and urine at 12.5%. The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 25,370 pg/mL in neat saliva (native) and 3903 pg/mL in neat urine (native).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human Apolipoprotein A-IV concentration was determined to be 192.8 µg/mL with a range of 9.2 – 418.0 µg/mL.

Protocols

References

This product has been referenced in:

  • Zeng J  et al. Increased serum protein levels by Yuanshi Shengmai Chenggu Tablet in treatment of avascular osteonecrosis of the femoral head. Mol Med Rep 17:2121-2126 (2018). Read more (PubMed: 29207081) »
See 1 Publication for this product

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