• Product name
    Human Apolipoprotein AI ELISA Kit (APOA1)
    See all Apolipoprotein A I kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.9%
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type
    Serum, Plasma
  • Assay type
  • Sensitivity
    1 µg/ml
  • Range
    0.625 µg/ml - 20 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.

    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.

    Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Human Apolipoprotein AI (Lyophilized) 1 vial
    Apolipoprotein AI Microplate (12 x 8 well strips) 1 unit
    Apolipoprotein AI Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • Tissue specificity
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • Involvement in disease
    High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • Sequence similarities
    Belongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • Apo-AI
    • ApoA-I
    • APOA1
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab108804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.


  • Apolipoprotein A1 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108804



This product has been referenced in:
  • Lin Q  et al. Decreased expression of the APOA1-APOC3-APOA4 gene cluster is associated with risk of Alzheimer's disease. Drug Des Devel Ther 9:5421-31 (2015). ELISA . Read more (PubMed: 26491253) »

See 1 Publication for this product

Customer reviews and Q&As

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Vielen Dank für Ihre Anfrage.

Leider haben wir kein weiteres Kit der Charge GR104136 mehr in unserem Lager (weltweit).

Diese Kits ist normalerweise sehr zuverlässig, aber Sie haben Recht, es gibt eine Komponente, die ni...

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Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit

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Abreview for Apolipoprotein A1 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2 µg
Type of Kit Used: ELISA
Positive control: recombinant apolipoprotein A1
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Jan 15 2013

Below is the information for the standard specific to your lot:

Standard Curve: Reconstitute the 120 mg of Apo A-I Standard with 3 ml of Diluent to generate a solution of 40 mg/ml. Allow the standard to sit for 10 minutes with gentle ag...

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I contacted the laboratory, and they responded that the Apo A-1 standard should be in micrograms, not nanograms. The current lot's standard is delivered at 60ug and 3mL of diluent would make the 20ug/ml solution. If you respond with your specific ...

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Vielen Dank für Ihre Anfrage. Ich entschuldige mich für die Wartezeit, ich musste auf nähere Angaben aus dem produzierenden Labor warten.

Ich freue mich Ihnen nun bestätigen zu können, dass beide Standards aus aufgere...

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Thank you for contacting Abcam.

After receiving the customer's complaint and abreview showing cross-reactivity with apoB using the kit ab108804 in May, we retested the kit for cross-reactivity. There was no significant cross-reactivity with ot...

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Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

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Thank you for your inquiry.
I am assuming that you are planning to use ab108804? https://www.abcam.com/index.html?datasheet=108804 https://www.abcam.com/index.html?datasheet=108804.
I can confirm that ab108804 has not been tested with cell cu...

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