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Customer kindly contacted us regarding the standard for ab108804. The standard is 120ng of lyopholized powder, and the protocol says to reconstitute in diluent to a concentration of 20ug/ml. Even if the entire 120ng was reconstituted at once, it would require only 6ul of diluent to make a 20ug/ml solution. Is this correct or is there a typo in the protocol?
Asked on Jan 14 2013
Below is the information for the standard specific to your lot:
Standard Curve: Reconstitute the 120 mg of Apo A-I Standard with 3 ml of Diluent to generate a solution of 40 mg/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the standard solution (40 mg/ml) 1:2 with equal volume Diluent to produce 20, 10, 5, 2.5, 1.25, and 0.625 mg/ml solutions. Diluent serves as the zero standard (0 mg/ml). Any remaining solution should be frozen at -200C.
I hope this information is helpful to you. Please do not hesitate to contact us with any further questions.
Answered on Jan 14 2013