Human Apolipoprotein AII Antibody Pair - BSA and Azide free (ab241659)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein AII Antibody Pair - BSA and Azide free
See all Apolipoprotein A II/ApoA-II kits -
Assay type
ELISA set -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Apolipoprotein A II. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab184859), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Apolipoprotein AII Capture Antibody (unconjugated) 1 x 100µg Human Apolipoprotein AII Detector Antibody (unconjugated) 1 x 100µg -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
Tissue specificity
Plasma; synthesized in the liver and intestine. -
Sequence similarities
Belongs to the apolipoprotein A2 family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APO A2
- Apo AII
- Apo-AII
see all -
Database links
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Images
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To learn more about the advantages of recombinant antibodies see here.
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Representative standard curve from corresponding SimpleStep ELISA® Kit (ab184859), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab241659 has not yet been referenced specifically in any publications.