Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.28 µg/ml
    • Range: 1 µg/ml - 8 µg/ml
    • Sample type: Cell culture extracts, Cell culture supernatant, Milk, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Apolipoprotein AII ELISA Kit (APOA2)
      See all Apolipoprotein A II/ApoA-II kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.7%
      Inter-assay
      Sample n Mean SD CV%
      Overall 9.9%

    • Sample type

      Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      = 0.28 µg/ml

    • Range

      1 µg/ml - 8 µg/ml

    • Recovery

      97 %

    • Assay time

      4h 00m

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in cell culture samples, serum and plasma, urine and milk.


      An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.


      Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    Associated products

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108805.

    References (1)

    Publishing research using ab108805? Please let us know so that we can cite the reference in this datasheet.

    ab108805 has been referenced in 1 publication.

    • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020

    Customer reviews and Q&As

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    Abreview for Apolipoprotein AII Human ELISA Kit

     Poor Poor 1/5 (Ease of Use)
    Abreviews
    abreview image

    Sample: Human
    Amount of Sample: 0-20 µg
    Type of Kit Used: ELISA
    Positive control: APOB, APOAII, HDL, LDL
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Kit detects APOB
    Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.

    Abcam user community

    Verified customer

    Submitted May 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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