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Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (1)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.28 µg/ml
    • Range: 1 µg/ml - 8 µg/ml
    • Sample type: Cell culture extracts, Cell culture supernatant, Milk, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Apolipoprotein AII ELISA Kit (APOA2)
      See all Apolipoprotein A II/ApoA-II kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.7%
      Inter-assay
      Sample n Mean SD CV%
      Overall 9.9%
    • Sample type

      Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      = 0.28 µg/ml
    • Range

      1 µg/ml - 8 µg/ml
    • Recovery

      97 %

    • Assay time

      4h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in cell culture samples, serum and plasma, urine and milk.


      An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.


      Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human Apolipoprotein AII Antibody 1 x 120µl
      Apolipoprotein AII Microplate (12 x 8 well strips) 1 unit
      Apolipoprotein AII Standard 2 vials
      Chromogen Substrate 1 x 7ml
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipid transport
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cancer proteins ELISA kits
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
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      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Heart disease
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Lipoprotein ELISA Kits
    • Function

      May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • Tissue specificity

      Plasma; synthesized in the liver and intestine.
    • Sequence similarities

      Belongs to the apolipoprotein A2 family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02652 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • APO A2
      • Apo AII
      • Apo-AII
      • APOA 2
      • ApoA II
      • ApoA-II
      • APOA2
      • APOA2_HUMAN
      • APOAII
      • Apolipoprotein A II
      • Apolipoprotein A-II(1-76)
      • Apolipoprotein A2
      • Apolipoprotein AII
      • ApolipoproteinA II
      • OTTHUMP00000032244
      see all
    • Database links

      • Entrez Gene: 336 Human
      • Omim: 107670 Human
      • SwissProt: P02652 Human
      • Unigene: 237658 Human

      Associated products

      • SimpleStep ELISA kits

        • Human Apolipoprotein AII ELISA Kit (ab184859)

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108805.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab108805? Please let us know so that we can cite the reference in this datasheet.

      ab108805 has been referenced in 1 publication.

      • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Abreview for Apolipoprotein AII Human ELISA Kit

      Poor Poor 1/5 (Ease of Use)
      Abreviews
      Abreviews
      abreview image

      Sample: Human
      Amount of Sample: 0-20 µg
      Type of Kit Used: ELISA
      Positive control: APOB, APOAII, HDL, LDL
      Did you use the Abcam Kit protocol?: Yes

      Additional data
      Additional Notes: Kit detects APOB
      Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.

      Abcam user community

      Verified customer

      Submitted May 04 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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