Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)
Key features and details
- Sensitivity: 0.28 µg/ml
- Range: 1 µg/ml - 8 µg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein AII ELISA Kit (APOA2)
See all Apolipoprotein A II/ApoA-II kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.7% Inter-assay Sample n Mean SD CV% Overall 9.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.28 µg/ml -
Range
1 µg/ml - 8 µg/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in plasma, serum, cell culture, cell lysates and tissue samples.
An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.
Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human Apolipoprotein AII Antibody 1 x 120µl Apolipoprotein AII Microplate (12 x 8 well strips) 1 unit Apolipoprotein AII Standard 2 vials Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
Tissue specificity
Plasma; synthesized in the liver and intestine. -
Sequence similarities
Belongs to the apolipoprotein A2 family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APO A2
- Apo AII
- Apo-AII
see all -
Database links
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
Associated products
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SimpleStep ELISA kits
Images
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Typical Standard Curve
Representative Standard Curve using ab108805.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108805 has been referenced in 1 publication.
- Niu R et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020