Product nameHuman Apolipoprotein B peptide
See all Apolipoprotein B proteins and peptides
Our Abpromise guarantee covers the use of ab199783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
- Apo B
- Apo B 100
- Apo B-100
FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in diseaseHypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
Sequence similaritiesContains 1 vitellogenin domain.
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
ab199783 has not yet been referenced specifically in any publications.