Overview

  • Product name

    Human Apolipoprotein CI ELISA Kit (APOCI)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.6%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.04 µg/ml
  • Range

    0.063 µg/ml - 4 µg/ml
  • Recovery

    98 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma, serum, cell lysates and cell culture supernatants.


    An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108808

Protocols

References

This product has been referenced in:

  • Yang J  et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). Read more (PubMed: 29754444) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can provide certificate of compliance which explain the kit will do what the datasheet say.

Once again I am sorry we will not be able to provide you free samples for testing. I can however provide Abtrial offer code if the kits are going to be used in samples or species not listed on the datasheet. By participating in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer. Please note that purchase for the first kit is necessary.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

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Question
Answer

I am sorry for the delay in getting back to you.

I can confirm that the antibodies used in this ELISA are polyclonal antibodies raised against the full length Apo CI protein.Theexact epitopes of binding are therefore not known and it is likely that there are multi-binding sites.

I am sorry that I could not be of more help in this instance, if you have any further questions please do not hesitate to contact us again.

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Question
Answer

Thank you for your call.

I can now confirm that in the kit with the lot number1239351 (amount is lotspecific)each vial contains 2 ug of Apo C-I standard.

We provide 2 vials of Apo C-I standard so the customer can use a fresh vial of standard when running another assay after some time.

Reconstitute the 2 ug of Apo C-I Standard with 0.5 ml of Diluent to generate a solution of 4 ug/ml. Allow the standard to sit on ice for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the standard solution (4 ug /ml) 1:2 with Diluent to produce 2, 1, 0.5, 0.25, 0.125 and 0.063 ug/ml solutions. Diluent serves as the zero standard (0 ug/ml). Any remaining solution should be frozen at -200C.

I hope this information is helpful. Please do not hesitate to contact me with any further questions.

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