Overview

  • Product name

    Human Apolipoprotein CIII ELISA Kit (APOC3)
    See all Apolipoprotein CIII kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell Lysate, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.3 ng/ml
  • Range

    0 µg/ml - 0.5 µg/ml
  • Recovery

    103 %

    Sample specific recovery
    Sample type Average % Range
    Serum 99.6 95% - 104%
    Plasma 98.6 94% - 103%

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Apolipoprotein CIII (APOC3) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab154131) is designed for the quantitative measurement of APOC3 in plasma, serum, urine, milk, CSF, cell lysate and cell culture samples.


    An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab154131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD).
  • APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • APOCIII detected in supernatants from cells after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab154131.

Protocols

References

This product has been referenced in:

  • Reyes-Soffer G  et al. Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism. Arterioscler Thromb Vasc Biol 39:63-72 (2019). Read more (PubMed: 30580564) »
  • Luo M  et al. Apolipoprotein CIII may mediate the impacts of angiopoietin-like protein 8 on triglyceride metabolism. Lipids Health Dis 17:160 (2018). Read more (PubMed: 30021607) »
See all 9 Publications for this product

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