Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameHuman Apolipoprotein CIII ELISA Kit (APOC3)
See all Apolipoprotein CIII kits -
Detection methodColorimetric
-
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma, Cell Lysate, Cerebral Spinal Fluid
-
Assay typeSandwich (quantitative)
-
Sensitivity0.3 ng/ml
-
Range0 µg/ml - 0.5 µg/ml
-
Recovery
103 %
Sample specific recovery Sample type Average % Range Serum 99.6 95% - 104% Plasma 98.6 94% - 103% -
Assay time4h 00m
-
Assay durationMultiple steps standard assay
-
Species reactivityReacts with: Human
-
Product overview
Human Apolipoprotein CIII (APOC3) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab154131) is designed for the quantitative measurement of APOC3 in plasma, serum, urine, milk, CSF, cell lysate and cell culture samples.
An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.
-
Tested applicationsSuitable for: Sandwich ELISAmore details
-
PlatformMicroplate
Properties
-
Storage instructionsStore at -20°C. Please refer to protocols.
-
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Apolipoprotein CIII Antibody 1 x 120µl Apolipoprotein CIII Microplate (12 x 8 well strips) 1 unit Apolipoprotein CIII Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
-
FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
-
Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
-
Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
-
Sequence similaritiesBelongs to the apolipoprotein C3 family.
-
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localizationSecreted.
- Information by UniProt
-
Alternative names
- APOC3
- APO C3
- Apo CIII
see all -
Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
Applications
Our Abpromise guarantee covers the use of ab154131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD). -
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD). -
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)APOCIII detected in supernatants from cells after background signal was subtracted (duplicates +/- SD). -
Typical Standard CurveRepresentative Standard Curve using ab154131.
Protocols
References
This product has been referenced in:
- Reyes-Soffer G et al. Effects of PCSK9 Inhibition With Alirocumab on Lipoprotein Metabolism in Healthy Humans. Circulation 135:352-362 (2017). Read more (PubMed: 27986651) »
- Millar JS et al. Effects of CETP inhibition with anacetrapib on metabolism of VLDL-TG and plasma apolipoproteins C-II, C-III, and E. J Lipid Res 58:1214-1220 (2017). Read more (PubMed: 28314859) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"