Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Human Apolipoprotein CIII ELISA Kit (APOC3)
See all Apolipoprotein CIII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell Lysate, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0 µg/ml - 0.5 µg/ml -
Recovery
103 %
Sample specific recovery Sample type Average % Range Serum 99.6 95% - 104% Plasma 98.6 94% - 103% -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Apolipoprotein CIII (APOC3) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab154131) is designed for the quantitative measurement of APOC3 in plasma, serum, urine, milk, CSF, cell lysate and cell culture samples.
An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Apolipoprotein CIII Antibody 1 x 120µl Apolipoprotein CIII Microplate (12 x 8 well strips) 1 unit Apolipoprotein CIII Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APOC3
- APO C3
- Apo CIII
see all -
Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
Applications
Our Abpromise guarantee covers the use of ab154131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD). -
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD). -
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)APOCIII detected in supernatants from cells after background signal was subtracted (duplicates +/- SD). -
Typical Standard CurveRepresentative Standard Curve using ab154131.
Protocols
References
This product has been referenced in:
- Reyes-Soffer G et al. Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism. Arterioscler Thromb Vasc Biol 39:63-72 (2019). Read more (PubMed: 30580564) »
- Luo M et al. Apolipoprotein CIII may mediate the impacts of angiopoietin-like protein 8 on triglyceride metabolism. Lipids Health Dis 17:160 (2018). Read more (PubMed: 30021607) »
