Human Apolipoprotein E ELISA Kit (APOE) (ab108813)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 0.016 µg/ml - 0.125 µg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein E ELISA Kit (APOE) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6.4% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 3 ng/ml -
Range
0.016 µg/ml - 0.125 µg/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Cynomolgus monkey -
Product overview
Human Apolipoprotein E ELISA kit (ApoE) is designed for the quantitative measurement of Apolipoprotein E concentrations in human plasma, serum, cerebrospinal fluid and cell culture supernatant samples.
An Apolipoprotein E specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein E specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein E captured in plate. The kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 1 x 30ml 1X Standard Diluent 1 x 2ml 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 2 x 30ml 30X Biotinylated Human Apolipoprotein E Antibody 1 vial 1 vial Apolipoprotein E Microplate (12 x 8 well strips) 1 unit 1 unit Apolipoprotein E Standard 2 vials 2 vials Chromogen Substrate 1 x 7ml 1 x 7ml Sealing Tapes 3 units 3 units Stop Solution 1 x 11ml 1 x 11ml -
Research areas
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Alternative names
- AD2
- Apo-E
- APOE
see all -
Database links
- Entrez Gene: 348 Human
- Omim: 107741 Human
- SwissProt: P02649 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab108813 has been referenced in 30 publications.
- Li M et al. Role of apolipoprotein E in suppressing oxidative stress in gestational diabetes mellitus patients and mouse model. Int J Gynaecol Obstet 159:204-212 (2022). PubMed: 34929050
- Bushweller L et al. Generation of Human Pluripotent Stem Cell-Derived Polarized Hepatocytes. Curr Protoc 2:e345 (2022). PubMed: 35007406
- Salazar IL et al. Posttranslational modifications of proteins are key features in the identification of CSF biomarkers of multiple sclerosis. J Neuroinflammation 19:44 (2022). PubMed: 35135578
- Colardo M et al. NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 35563230
- Sepulveda J et al. Independent APOE4 knock-in mouse models display reduced brain APOE protein, altered neuroinflammation, and simplification of dendritic spines. J Neurochem 163:247-259 (2022). PubMed: 35838553