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    human-apoptosis-antibody-array-membrane-43-targets-ab134001.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a question References (28)

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Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets)

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    Overview

    • Product name

      Human Apoptosis Antibody Array - Membrane (43 Targets)
    • Sample type

      Tissue Extracts, Cell Lysate
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.


      Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP


      Caspase 3-will detect only the active form


      Caspase 8-will detect the full length pro-form and the active form.


      Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


      Learn more about membrane antibody arrays

    • Notes

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Tested applications

      Suitable for: Multiplex Protein Detectionmore details

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
      1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl
      1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
      20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
      20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
      2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml
      8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
      Apoptosis Antibody Array Membranes 4 units 8 units 2 units
      Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial
      Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
      Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
      Protease Inhibitor Cocktail 1 vial 2 vials 1 vial
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Bcl2 Family
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • p53 pathway
      • Antibody Arrays
      • Membrane antibody arrays
      • Apoptosis antibody arrays
      • Antibody Arrays
      • Membrane antibody arrays
      • EGFR phosphorylation antibody arrays

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab134001 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.
    Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.

    Images

    • Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets)
      Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets)

      Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines

      107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (28)

    Publishing research using ab134001? Please let us know so that we can cite the reference in this datasheet.

    ab134001 has been referenced in 28 publications.

    • Huang Y  et al. GSG2 Promotes Development and Predicts Poor Prognosis of Ovarian Cancer. Cancer Manag Res 13:499-508 (2021). PubMed: 33500663
    • Wang X  et al. Double-targeting CDCA8 and E2F1 inhibits the growth and migration of malignant glioma. Cell Death Dis 12:146 (2021). PubMed: 33542211
    • Lai DM  et al. CCNI2 plays a promoting role in the progression of colorectal cancer. Cancer Med 10:1913-1924 (2021). PubMed: 33620152
    • Zhang C  et al. Targeting POLE2 Creates a Novel Vulnerability in Renal Cell Carcinoma via Modulating Stanniocalcin 1. Front Cell Dev Biol 9:622344 (2021). PubMed: 33644060
    • Feng Q  et al. CDC42EP3 promotes colorectal cancer through regulating cell proliferation, cell apoptosis and cell migration. Cancer Cell Int 21:169 (2021). PubMed: 33726765
    View all Publications for this product

    Customer reviews and Q&As

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