Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Human Apoptosis Antibody Array - Membrane (43 Targets) -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.
Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP
Caspase 3-will detect only the active form
Caspase 8-will detect the full length pro-form and the active form.
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Apoptosis Antibody Array Membranes 4 units 8 units 2 units Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Protease Inhibitor Cocktail 1 vial 2 vials 1 vial -
Research areas
Applications
Our Abpromise guarantee covers the use of ab134001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets)Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines
107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.
Protocols
References
This product has been referenced in:
- Maity R et al. Gold nanoparticle-assisted enhancement in the anti-cancer properties of theaflavin against human ovarian cancer cells. Mater Sci Eng C Mater Biol Appl 104:109909 (2019). Human . Read more (PubMed: 31499983) »
- Zhao L et al. TERT assists GDF11 to rejuvenate senescent VEGFR2+/CD133+ cells in elderly patients with myocardial infarction. Lab Invest 99:1661-1688 (2019). Read more (PubMed: 31292540) »
