Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)
Overview
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Product name
Human Apoptosis Antibody Array - Membrane (43 Targets) -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.
Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP
Caspase 3-will detect only the active form
Caspase 8-will detect the full length pro-form and the active form.
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Apoptosis Antibody Array Membranes 4 units 8 units 2 units Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Protease Inhibitor Cocktail 1 vial 2 vials 1 vial -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| Notes |
|---|
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Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets)
Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines
107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab134001 has been referenced in 28 publications.
- Huang Y et al. GSG2 Promotes Development and Predicts Poor Prognosis of Ovarian Cancer. Cancer Manag Res 13:499-508 (2021). PubMed: 33500663
- Wang X et al. Double-targeting CDCA8 and E2F1 inhibits the growth and migration of malignant glioma. Cell Death Dis 12:146 (2021). PubMed: 33542211
- Lai DM et al. CCNI2 plays a promoting role in the progression of colorectal cancer. Cancer Med 10:1913-1924 (2021). PubMed: 33620152
- Zhang C et al. Targeting POLE2 Creates a Novel Vulnerability in Renal Cell Carcinoma via Modulating Stanniocalcin 1. Front Cell Dev Biol 9:622344 (2021). PubMed: 33644060
- Feng Q et al. CDC42EP3 promotes colorectal cancer through regulating cell proliferation, cell apoptosis and cell migration. Cancer Cell Int 21:169 (2021). PubMed: 33726765