Product nameHuman Aquaporin 4 peptide
See all Aquaporin 4 proteins and peptides
Our Abpromise guarantee covers the use of ab46181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Aquaporin 4 antibody (ab46182)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- AQP 4
FunctionForms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Tissue specificityBrain - muscle >> heart, kidney, lung, and trachea.
Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
modificationsPhosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab46181 has been referenced in 1 publication.
- Hemley SJ et al. Aquaporin-4 expression in post-traumatic syringomyelia. J Neurotrauma 30:1457-67 (2013). WB ; Rat . PubMed: 23441695