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    human-arid1a-knockout-hek-293t-cell-pellet-ab278824.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors
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Human ARID1A knockout HEK-293T cell pellet (ab278824)

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Western blot - Human ARID1A knockout HEK293T cell pellet (ab278824)
  • Sanger Sequencing - Human ARID1A knockout HEK293T cell pellet (ab278824)

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Anti-ARID1A antibody [EPR13501] (ab182560)
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Human ARID1A knockout HEK-293T cell lysate (ab257250)
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Overview

  • Product name

    Human ARID1A knockout HEK-293T cell pellet
    See all ARID1A kits
  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1.
  • Passage number

    <20
  • Knockout validation

    Sanger Sequencing
  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WB, Sanger Sequencingmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab286210 - Human ARID1A knockout HEK293T cell pellet 1 vial
    ab286211 - Human wild-type HEK293T cell pellet 1 vial
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity

    Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similarities

    Contains 1 ARID domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O14497 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • actin-dependent regulator of chromatin subfamily F member 1
    • ARI1A_HUMAN
    • ARID domain containing protein 1A
    • ARID domain-containing protein 1A
    • ARID1A
    • AT rich interactive domain 1A
    • AT rich interactive domain 1A (SWI like)
    • AT rich interactive domain containing protein 1A
    • AT-rich interactive domain-containing protein 1A
    • B120
    • BAF250
    • BAF250A
    • BM029
    • brain protein 120
    • BRG1 associated factor 250
    • BRG1 associated factor 250a
    • BRG1-associated factor 250
    • BRG1-associated factor 250a
    • C1ORF4
    • chromatin remodeling factor p250
    • chromosome 1 open reading frame 4
    • ELD
    • hELD
    • hOSA1
    • matrix-associated
    • MRD14
    • Osa homolog 1
    • OSA1
    • OSA1 nuclear protein
    • P270
    • SMARCF1
    • SWI like protein
    • SWI SNF complex protein p270
    • SWI-like protein
    • SWI/SNF complex protein p270
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1
    • SWI/SNF-related
    see all

Associated products

  • KO cell lines

    • Human ARID1A knockout HEK-293T cell line (ab266189)
  • KO cell lysates

    • Human ARID1A knockout HEK-293T cell lysate (ab257250)
  • Related Products

    • Anti-ARID1A antibody [EPR13501] (ab182560)
    • Anti-ARID1A antibody [EPR13501] - BSA and Azide free (ab217154)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 242 kDa.
Sanger Sequencing
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 242 kDa.
Sanger Sequencing
Use at an assay dependent concentration.

Images

  • Western blot - Human ARID1A knockout HEK293T cell pellet (ab278824)
    Western blot - Human ARID1A knockout HEK293T cell pellet (ab278824)

    Lane 1: Wild-type HEK-293T cell lysate 20 ug
    Lane 2: ARID1A knockout HEK-293T cell lysate 20 ug
    Lane 3: SH-SY5Y cell lysate 20 ug
    Lanes 1 - 3:Merged signal (red and green). Green - ab182560 observed at 270 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
    ab182560 was shown to react with ARID1A in wild-type HEK-293T cells in Western blot with loss of signal observed in ARID1A knockout cell line ab260928 (ARID1A knockout cell lysate ab257250). Wild-type HEK-293T and ARID1A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab182560 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Sanger Sequencing - Human ARID1A knockout HEK293T cell pellet (ab278824)
    Sanger Sequencing - Human ARID1A knockout HEK293T cell pellet (ab278824)
    Homozygous: 11 bp deletion in exon1

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab278824? Please let us know so that we can cite the reference in this datasheet.

ab278824 has not yet been referenced specifically in any publications.

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