Overview

  • Product name

    Human Artemin ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 8 100pg/ml 1.99%
    2 8 460pg/ml 1.23%
    3 8 1830pg/ml 4.57%
    Inter-assay
    Sample n Mean SD CV%
    1 3 110pg/ml 9.09%
    2 3 450pg/ml 8.91%
    3 3 1910pg/ml 5.54%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2 pg/ml
  • Range

    30 pg/ml - 1920 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 107.88 0pg/ml - 960pg/ml

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The human Artemin ELISA kit (ab215416) is designed for the quantitative determination of human Artemin in cell culture supernatant, serum and plasma.


     

  • Notes

    Microplate wells are pre-coated with anti-human Artemin-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human Artemin molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human Artemin-specific monoclonal detection antibodies bind to the same epitope on another molecule of dimeric human Artemin. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the color produced is directly proportional to the amount of human Artemin in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash concentrate 1 x 50ml
    Anti-hARTN coated microplate (12 x 8 wells) 1 unit
    hARTN enzyme conjugate 1 x 12ml
    hARTN sample diluent 1 x 25ml
    hARTN standard stock solution 2 x 30µl
    Stop solution 1 x 12ml
    TMB Substrate solution 1 x 12ml
  • Research areas

  • Function

    Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
  • Tissue specificity

    Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.
  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.
  • Developmental stage

    Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Artemin
    • Artn
    • ARTN_HUMAN
    • Enovin
    • EVN
    • NBN
    • Neublastin
    • Neurotrophic factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab215416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Cubic spline fit curve on a log-log scale.

  • Linear regression (LLS) fit curve on a lin-lin scale.

     

Protocols

References

ab215416 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215416.
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