Overview

  • Product name

    Human Axl ELISA Kit
    See all Axl kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    2.74 pg/ml - 2000 pg/ml
  • Recovery

    < 94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.35 82% - 102%
    Serum 91.77 82% - 102%
    Plasma 93.49 83% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Axl Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Axl in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Axl coated on a 96-well plate. Standards and samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Axl antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99976.

  • Representative Standard Curve using ab99976.

Protocols

References

This product has been referenced in:

  • Xu J  et al. Identification of the soluble form of tyrosine kinase receptor Axl as a potential biomarker for intracranial aneurysm rupture. BMC Neurol 15:23 (2015). Human . Read more (PubMed: 25885003) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. I have had the opportunity to discuss your question with the lab. They have informed me that the antibody pair in the ab99976 recognizes a region on the extracellular domain. Therefore the soluble form of this receptor is being detected. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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