Overview

  • Product name

    Human BAFF ELISA Kit (TNFSF13B)
    See all BAFF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 8.8%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    12.7 pg/ml
  • Range

    0.08 ng/ml - 5 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 105% - 115%
    Cell culture media 108 106% - 110%
    Heparin Plasma 104 101% - 108%
    EDTA Plasma 105 98% - 115%
    Citrate Plasma 106 100% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    BAFF (TNFSF13B) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BAFF protein in human serum and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    B-cell activating factor (BAFF) protein that is encoded by the TNFSF13B gene. BAFF protein is expressed in B-cell lineage cells which functions as a cytokine and is also a member of the tumor necrosis factor (TNF) family of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. This protein is expressed as a membrane-bound type II transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The membrane bound form can be cleaved from the membrane, generating a soluble protein fragment. BAFF steady-state concentrations depend on B-cells and also on the expression of BAFF-binding receptors.

    Inadequate levels of BAFF will fail to activate B-cells to produce enough immunoglobulin and will lead to immunodeficiency. Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythmatosis, rheumatoid arthritis, and many other autoimmune diseases.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human BAFF Capture Antibody 1 x 600µl
    10X Human BAFF Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human BAFF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
  • Tissue specificity

    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
    N-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • B lymphocyte stimulator
    • B-cell-activating factor
    • BAFF
    • BLyS
    • CD257
    • Dendritic cell-derived TNF-like molecule
    • soluble form
    • TALL-1
    • TN13B_HUMAN
    • TNF- and APOL-related leukocyte expressed ligand 1
    • TNFSF13B
    • Tumor necrosis factor ligand superfamily member 13b
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab188391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Bar graph denotes interpolated values of BAFF protein across dilution series.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Bar graph denotes interpolated values of BAFF protein across dilution series.

  • Quantification of BAFF interpolated from standard curve and multiplied by dilution factor.

Protocols

References

This product has been referenced in:

  • Alkhairy OK  et al. RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency. J Allergy Clin Immunol N/A:N/A (2014). ELISA ; Human . Read more (PubMed: 25512081) »
See 1 Publication for this product

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