Human BAFF-R ELISA Kit (ab213839)
Overview
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Product name
Human BAFF-R ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 643pg/ml 29.6 4.6% 2 16 1604pg/ml 57.75 3.6% 3 16 3262pg/ml 169.6 5.2% Inter-assay Sample n Mean SD CV% 1 24 745pg/ml 43.21 5.8% 2 24 1862pg/ml 91.3 4.9% 3 24 3657pg/ml 234.1 6.4% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.
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Notes
BAFF-R (also known as Tumor necrosis factor receptor superfamily member 13C or B-cell-activating factor receptor) is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human BAFF-R coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human BAFF-R antibody 1 x 130µl Lyophilized Recombinant Human BAFF-R Standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes. -
Involvement in disease
Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B cell activating factor receptor
- B-cell-activating factor receptor
- BAFF R
see all -
Database links
- Entrez Gene: 115650 Human
- Omim: 606269 Human
- SwissProt: Q96RJ3 Human
- Unigene: 344088 Human
Images
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Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.
References
ab213839 has not yet been referenced specifically in any publications.
