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Signal Transduction Growth Factors/Hormones TNF
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Human BAFF-R ELISA Kit (ab213839)

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Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 78 pg/ml - 5000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Recombinant human BAFF-R protein (ab157066)

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    Overview

    • Product name

      Human BAFF-R ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 147pg/ml 10.29 = 7%
      2 16 683pg/ml 49.85 = 7.3%
      3 16 2750pg/ml 206.25 = 7.5%
      Inter-assay
      Sample n Mean SD CV%
      1 24 156pg/ml 13.88 = 8.9%
      2 24 700pg/ml 62.3 = 8.9%
      3 24 2531pg/ml 227.05 = 8.9%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      78 pg/ml - 5000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


      The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

    • Notes

      BAFF-R (also known as Tumor necrosis factor receptor superfamily member 13C or B-cell-activating factor receptor)  is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human BAFF-R coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human BAFF-R antibody 1 x 130µl
      Lyophilized Recombinant Human BAFF-R Standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Signal Transduction
      • Growth Factors/Hormones
      • TNF
    • Function

      B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
    • Tissue specificity

      Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.
    • Involvement in disease

      Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities

      Contains 1 TNFR-Cys repeat.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q96RJ3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • B cell activating factor receptor
      • B-cell-activating factor receptor
      • BAFF R
      • BAFF receptor
      • BAFF-R
      • BAFFR
      • BLyS receptor 3
      • BlySR3
      • BR 3
      • BR3
      • BROMIX
      • CD 268
      • CD268
      • CD268 antigen
      • CVID4
      • MGC138235
      • OTTHUMP00000028746
      • Prolixin
      • TNFRSF 13C
      • Tnfrsf13c
      • TR13C_HUMAN
      • Tumor necrosis factor receptor subunit member 13C
      • Tumor necrosis factor receptor superfamily member 13C
      see all
    • Database links

      • Entrez Gene: 115650 Human
      • Omim: 606269 Human
      • SwissProt: Q96RJ3 Human
      • Unigene: 344088 Human

      Associated products

        Images

        • Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.
          Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.

          Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (0)

        Publishing research using ab213839? Please let us know so that we can cite the reference in this datasheet.

        ab213839 has not yet been referenced specifically in any publications.

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