Human BAG3 ELISA Kit (ab227900)
- Datasheet
- References
- Protocols
Overview
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Product name
Human BAG3 ELISA Kit
See all Bag3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HEK-293T 5 5.2% Inter-assay Sample n Mean SD CV% HEK-293T 3 2.5% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
11.7 pg/well -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 97 93% - 99% Tissue Extracts 104 103% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Human -
Product overview
BAG3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Bag3 protein in human cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BAG3 Capture Antibody 1 x 600µl 10X Human BAG3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BAG3 Lyophilized Recombinant Protein 1 x 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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Alternative names
- BAG 3
- BAG family molecular chaperone regulator 3
- BAG-3
see all -
Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab227900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human BAG3 ELISA Kit (ab227900)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of humanBag3 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 µg/mL extract loadThe concentrations of Bag3 were measured in duplicate and interpolated from the Bag3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bag3 concentration was determined to be 2497 pg/mL in HEK293T cell extract and 2743 pg/mL in human heart tissue extract.
Protocols
References
ab227900 has not yet been referenced specifically in any publications.
