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    human-bag3-elisa-kit-ab227900.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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RecombinantSimpleStep

Human BAG3 ELISA Kit (ab227900)

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  • SDS
  • Protocol Booklet
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Other - Human BAG3 ELISA Kit (ab227900)
  • Example of humanBag3 standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 µg/mL extract load
  • Sandwich ELISA - Human BAG3 ELISA Kit (ab227900)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11.7 pg/well
  • Range: 39.1 pg/ml - 2500 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human BAG3 ELISA Kit
    See all Bag3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HEK-293T 5 5.2%
    Inter-assay
    Sample n Mean SD CV%
    HEK-293T 3 2.5%
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.7 pg/well
  • Range

    39.1 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 97 93% - 99%
    Tissue Extracts 104 103% - 105%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human BAG3 ELISA Kit (ab227900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAG3 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BAG3 with 11.7 pg/well sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human BAG3 Capture Antibody 1 x 600µl
    10X Human BAG3 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human BAG3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
  • Function

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in disease

    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similarities

    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Target information above from: UniProt accession O95817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • BAG 3
    • BAG family molecular chaperone regulator 3
    • BAG-3
    • Bag3
    • BAG3_HUMAN
    • Bcl 2 binding protein
    • Bcl-2-associated athanogene 3
    • Bcl-2-binding protein Bis
    • BCL2 associated athanogene 3
    • BCL2 binding athanogene 3
    • BIS
    • CAIR 1
    • Docking protein CAIR 1
    • Docking protein CAIR-1
    • MFM6
    see all
  • Database links

    • Entrez Gene: 9531 Human
    • Omim: 603883 Human
    • SwissProt: O95817 Human
    • Unigene: 523309 Human

    Associated products

    • Alternative Versions

      • Human BAG3 Antibody Pair - BSA and Azide free (ab244047)

    Images

    • Other - Human BAG3 ELISA Kit (ab227900)
      Other - Human BAG3 ELISA Kit (ab227900)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of humanBag3 standard curve in 1X Cell Extraction Buffer PTR
      Example of humanBag3 standard curve in 1X Cell Extraction Buffer PTR

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 µg/mL extract load
      Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 µg/mL extract load

      The concentrations of Bag3 were measured in duplicate and interpolated from the Bag3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bag3 concentration was determined to be 2497 pg/mL in HEK293T cell extract and 2743 pg/mL in human heart tissue extract.

    • Sandwich ELISA - Human BAG3 ELISA Kit (ab227900)
      Sandwich ELISA - Human BAG3 ELISA Kit (ab227900)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab227900? Please let us know so that we can cite the reference in this datasheet.

    ab227900 has not yet been referenced specifically in any publications.

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