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|Sample type||Average %||Range|
|Serum||= 76.07||68% - 86%|
|Plasma||= 86.57||80% - 94%|
|Cell Lysate||= 84.81||76% - 92%|
Abcam’s BCAM (Basal Cell Adhesion Molecule) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCAM in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human BCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and BCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|240X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|BCAM-coated Microplate||1 unit|
|Biotinylated anti-Human BCAM||2 vials|
|Recombinant Human BCAM Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155425 has not yet been referenced specifically in any publications.