Overview

  • Product name
    Human Bcl-2 ELISA Kit
    See all Bcl-2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12%
  • Sample type
    Serum, Plasma, Cell culture extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.5 ng/ml
  • Range
    0.5 ng/ml - 32 ng/ml
  • Recovery

    71 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.


    Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.


    Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 70µl
    Human Bcl-2 Standard lyophilized (64 ng/mL upon reconstitution) 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Microplate coated with monoclonal antibody to Bcl-2 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Tissue specificity
    Expressed in a variety of tissues.
  • Involvement in disease
    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • Post-translational
    modifications
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • Cellular localization
    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Alternative names
    • Apoptosis regulator Bcl 2
    • Apoptosis regulator Bcl-2
    • Apoptosis regulator Bcl2
    • AW986256
    • B cell CLL/lymphoma 2
    • B cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl2
    • BCL2_HUMAN
    • C430015F12Rik
    • D630044D05Rik
    • D830018M01Rik
    • Leukemia/lymphoma, B-cell, 2
    • Oncogene B-cell leukemia 2
    • PPP1R50
    • Protein phosphatase 1, regulatory subunit 50
    see all
  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab119506 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119506.

    Protocols

    References

    This product has been referenced in:
    • Lu HJ  et al. Mechanism of MicroRNA-708 Targeting BAMBI in Cell Proliferation, Migration, and Apoptosis in Mice With Melanoma via the Wnt and TGF-ß Signaling Pathways. Technol Cancer Res Treat 17:1533034618756784 (2018). Read more (PubMed: 29466930) »
    • Deng J  et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother 98:102-110 (2018). WB ; Human . Read more (PubMed: 29247949) »

    See all 3 Publications for this product

    Publishing research using ab119506? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    I am interested in performing an ELISA with your BCL-2 Human Antibody. Approximately which region of the protein does this antibody recognize? Has the binding been tested to the native protein conformation or denatured protein only?

    While we do not have binding information available, AB119506, BML2 assay, detected Bcl-2 in pathological sera from patients with melanoma. The originating lab also performed protein spike recovery assays.


    Inquiry: Hi! I have a question concerning the BCL-2 ELISA kit ab119506. Could you please tell me, which BCL-2 isoform is detected with the kit (alpha or beta). It would also help me, if you could tell me against which epitopes the antibodies are d...

    Read More

    Hello!

    This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

    Please let me know if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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