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    human-bcl-2-elisa-kit-ab119506.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Human Bcl-2 ELISA Kit (ab119506)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (9)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.5 ng/ml
    • Range: 0.5 ng/ml - 32 ng/ml
    • Sample type: Cell culture extracts, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Bcl-2 ELISA Kit
      See all Bcl-2 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 8.6%
      Inter-assay
      Sample n Mean SD CV%
      Overall 12%
    • Sample type

      Serum, Plasma, Cell culture extracts
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.5 ng/ml
    • Range

      0.5 ng/ml - 32 ng/ml
    • Recovery

      71 %

    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.


      Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.


      Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Assay Buffer Concentrate 1 x 5ml
      20X Wash Buffer Concentrate 1 x 50ml
      Adhesive Films 4 units
      Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 70µl
      Human Bcl-2 Standard lyophilized (64 ng/mL upon reconstitution) 2 vials
      Lysis Buffer (10x) 1 x 15ml
      Microplate coated with monoclonal antibody to Bcl-2 (12 x 8 wells) 1 unit
      Sample Diluent 1 x 12ml
      Stop Solution (1M Phosphoric acid) 1 x 15ml
      Streptavidin-HRP 1 x 150µl
      TMB Substrate Solution 1 x 15ml
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Bcl2 Family
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Bcl 2 family
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Cell survival & death
      • Cancer
      • Tumor biomarkers
      • Oncoproteins
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Bcl 2 family
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism
    • Function

      Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
    • Tissue specificity

      Expressed in a variety of tissues.
    • Involvement in disease

      A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Domain

      BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
      The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
      The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
    • Post-translational
      modifications

      Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
      Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
      Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
    • Cellular localization

      Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P10415 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Apoptosis regulator Bcl 2
      • Apoptosis regulator Bcl-2
      • Apoptosis regulator Bcl2
      • AW986256
      • B cell CLL/lymphoma 2
      • B cell leukemia/lymphoma 2
      • Bcl-2
      • Bcl2
      • BCL2_HUMAN
      • C430015F12Rik
      • D630044D05Rik
      • D830018M01Rik
      • Leukemia/lymphoma, B-cell, 2
      • Oncogene B-cell leukemia 2
      • PPP1R50
      • Protein phosphatase 1 regulatory subunit 50
      • Protein phosphatase 1, regulatory subunit 50
      see all
    • Database links

      • Entrez Gene: 596 Human
      • Omim: 151430 Human
      • SwissProt: P10415 Human
      • Unigene: 150749 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab119506.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (9)

        Publishing research using ab119506? Please let us know so that we can cite the reference in this datasheet.

        ab119506 has been referenced in 9 publications.

        • Abo-Salem HM  et al. Synthesis and Bioactivity Assessment of Novel Spiro Pyrazole-Oxindole Congeners Exhibiting Potent and Selective in vitro Anticancer Effects. Molecules 25:N/A (2020). PubMed: 32138244
        • Abu-Zaied MA  et al. Novel purine thioglycoside analogs: synthesis, nanoformulation and biological evaluation in in vitro human liver and breast cancer models. Drug Des Devel Ther 13:2437-2457 (2019). PubMed: 31440030
        • Zheng YJ  et al. Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1. Mol Ther Nucleic Acids 18:485-495 (2019). PubMed: 31670198
        • Yao W  et al. Over-expressed LOC101927196 suppressed oxidative stress levels and neuron cell proliferation in a rat model of autism through disrupting the Wnt signaling pathway by targeting FZD3. Cell Signal 62:109328 (2019). PubMed: 31145996
        • Chen FZ  et al. MicroRNA-98 reduces amyloid ß-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. Int J Mol Med 43:91-102 (2019). PubMed: 30365070
        • Chen W  et al. Effects of Long Non-Coding RNA LINC00963 on Renal Interstitial Fibrosis and Oxidative Stress of Rats with Chronic Renal Failure via the Foxo Signaling Pathway. Cell Physiol Biochem 46:815-828 (2018). PubMed: 29627834
        • Lu HJ  et al. Mechanism of MicroRNA-708 Targeting BAMBI in Cell Proliferation, Migration, and Apoptosis in Mice With Melanoma via the Wnt and TGF-ß Signaling Pathways. Technol Cancer Res Treat 17:1533034618756784 (2018). PubMed: 29466930
        • Deng J  et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother 98:102-110 (2018). WB ; Human . PubMed: 29247949
        • Rayavarapu S  et al. Synthesis of Saccharumoside-B analogue with potential of antiproliferative and pro-apoptotic activities. Sci Rep 7:8309 (2017). PubMed: 28814788

        Customer reviews and Q&As

        Show All Reviews Q&A
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        1-2 of 2 Abreviews or Q&A

        Question

        I am interested in performing an ELISA with your BCL-2 Human Antibody. Approximately which region of the protein does this antibody recognize? Has the binding been tested to the native protein conformation or denatured protein only?

        Read More

        Abcam community

        Verified customer

        Asked on Jan 16 2013

        Answer

        While we do not have binding information available, AB119506, BML2 assay, detected Bcl-2 in pathological sera from patients with melanoma. The originating lab also performed protein spike recovery assays.

        Read More

        Abcam Scientific Support

        Answered on Jan 17 2013

        Question


        Inquiry: Hi! I have a question concerning the BCL-2 ELISA kit ab119506. Could you please tell me, which BCL-2 isoform is detected with the kit (alpha or beta). It would also help me, if you could tell me against which epitopes the antibodies are directed.

        Read More

        Abcam community

        Verified customer

        Asked on Oct 30 2012

        Answer

        Hello!

        This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

        Please let me know if you have any further questions.

        Read More

        Abcam Scientific Support

        Answered on Oct 30 2012

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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