Overview

  • Product name

    Human Bcl-2 ELISA Kit
    See all Bcl-2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12%
  • Sample type

    Serum, Plasma, Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.5 ng/ml
  • Range

    0.5 ng/ml - 32 ng/ml
  • Recovery

    71 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.


    Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.


    Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 70µl
    Human Bcl-2 Standard lyophilized (64 ng/mL upon reconstitution) 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Microplate coated with monoclonal antibody to Bcl-2 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas

  • Function

    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Tissue specificity

    Expressed in a variety of tissues.
  • Involvement in disease

    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • Post-translational
    modifications

    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • Cellular localization

    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Alternative names

    • Apoptosis regulator Bcl 2
    • Apoptosis regulator Bcl-2
    • Apoptosis regulator Bcl2
    • AW986256
    • B cell CLL/lymphoma 2
    • B cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl2
    • BCL2_HUMAN
    • C430015F12Rik
    • D630044D05Rik
    • D830018M01Rik
    • Leukemia/lymphoma, B-cell, 2
    • Oncogene B-cell leukemia 2
    • PPP1R50
    • Protein phosphatase 1, regulatory subunit 50
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab119506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119506.

Protocols

References

This product has been referenced in:

  • Chen FZ  et al. MicroRNA-98 reduces amyloid ß-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. Int J Mol Med 43:91-102 (2019). Read more (PubMed: 30365070) »
  • Lu HJ  et al. Mechanism of MicroRNA-708 Targeting BAMBI in Cell Proliferation, Migration, and Apoptosis in Mice With Melanoma via the Wnt and TGF-ß Signaling Pathways. Technol Cancer Res Treat 17:1533034618756784 (2018). Read more (PubMed: 29466930) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

While we do not have binding information available, AB119506, BML2 assay, detected Bcl-2 in pathological sera from patients with melanoma. The originating lab also performed protein spike recovery assays.

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Answer

Hello!

This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

Please let me know if you have any further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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