SimpleStep

Human BDNF ELISA Kit (ab212166)

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Overview

  • Product name
    Human BDNF ELISA Kit
    See all BDNF kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.3%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.4 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 103% - 109%
    Serum 97 94% - 104%
    Heparin Plasma 103 96% - 112%
    EDTA Plasma 98 85% - 108%
    Citrate Plasma 103 95% - 111%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Human BDNF in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of free BDNF in human serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    BDNF (Brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab212166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Other - Human BDNF ELISA Kit (ab212166)
    Other - Human BDNF ELISA Kit (ab212166)
    ELISA Protocol Summary
  • Example of human BDNF standard curve.
    Example of human BDNF standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native BDNF in human serum, and plasma samples
    Interpolated concentrations of native BDNF in human serum, and plasma samples

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).

  • Serum from ten individual healthy human male and female donors was measured in duplicate.
    Serum from ten individual healthy human male and female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.

  • Interpolated concentrations of native BDNF in rat serum sample.
    Interpolated concentrations of native BDNF in rat serum sample.

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.

  • Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.
    Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.

    L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.

Protocols

References

This product has been referenced in:
  • Lee HL  et al. Yuk-Mi-Jihwang-Tang , a Traditional Korean Multiple Herbal Formulae, Improves Hippocampal Memory on Scopolamine Injection-Induced Amnesia Model of C57BL/6 Mice. Evid Based Complement Alternat Med 2018:2821040 (2018). Read more (PubMed: 29507588) »
  • Grasman JM & Kaplan DL Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves. Sci Rep 7:4092 (2017). ELISA . Read more (PubMed: 28642578) »
See all 2 Publications for this product

Publishing research using ab212166? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

ab212166 - Human BDNF ELISA Kit-Use in marmoset plasma

 Inconclusive Good 4/5 (Ease of Use)
Abreviews
abreview image
I have used the BDNF ELISA kit on proteins isolated from marmoset plasma(heparin). The samples were undiluted and diluted 1:2 and 1:4. I did not detect the BDNF in the sample of marmoset. However, I was able to detect in the same condition with the rat plasma. And, I tried to lengthen discrimination time, but was not able to detect in the plasma of the marmoset. I think that the BDNF ELISA kit ab212166 can not work for BDNF detection in marmoset sample.

Abcam user community

Verified customer

Submitted Nov 04 2016

ab212166 - Human BDNF ELISA Kit-Use in rats brain tissue

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I have used the BDNF elisa kit on proteins isolated from rat brain (hippocampus). In order to create the same conditions in both standard and samples i add 100 micro-liters of lysis buffer to the standard stock (lysis buffer- 200mM EDTA, 100mM tris,IGEPAL,Protease Inhibitor and Phosphatase Inhibitor in DDW). The samples were diluted 1:10 in order to minimize lysis buffer interference with the assay. The detection of BDNF in the samples was good as well as the standard curve which was accurate (despite the lysis buffer ). I think that the BDNF elisa kit ab212166 can defiantly work for BDNF detection in brain tissue lysats

Ravit Uzan

Verified customer

Submitted Jul 06 2016

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