SimpleStep

Human BDNF ELISA Kit (ab212166)

Reviews (2) Submit a question References (6)
Other - Human BDNF ELISA Kit (ab212166)
  • Example of human BDNF standard curve.
  • Example of human BDNF standard curve in Sample Diluent NS.
  • Interpolated concentrations of native BDNF in human serum, and plasma samples
  • Serum from ten individual healthy human male and female donors was measured in duplicate.
  • Interpolated concentrations of native BDNF in rat serum sample.
  • Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.
  • Linearity of dilution in Sample Diluent NS.
  • Linearity of dilution in Sample Diluent NS.
  • Linearity of dilution in Sample Diluent NS.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.4 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human BDNF ELISA Kit
    See all BDNF kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.3%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.4 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 103% - 109%
    Serum 97 94% - 104%
    Hep Plasma 103 96% - 112%
    EDTA Plasma 98 85% - 108%
    Cit plasma 103 95% - 111%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human BDNF ELISA Kit (ab212166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BDNF protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BDNF with 2.4 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    SPECIES REACTIVITY


    This kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein. 


    ASSAY SPECIFICITY


    This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only.


    Cell and tissue extract samples have not been tested with this kit.


    CROSS REACTIVITY


    Recombinant TRKB was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity.  No cross-reactivity was observed.


    INTERFERENCE


    Recombinant TRKB was prepared at 50 ng/mL and 1 ng/mL and tested for interference with human BDNF at 200 pg/mL. 39% Inference was observed with 1ng/mL and 76% Interference was observed with 50 ng/mL.

  • Notes

    BDNF (Brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human BDNF ELISA Kit (ab212166)
    Other - Human BDNF ELISA Kit (ab212166)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human BDNF standard curve.
    Example of human BDNF standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human BDNF standard curve in Sample Diluent NS.
    Example of human BDNF standard curve in Sample Diluent NS.

    The BDNF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native BDNF in human serum, and plasma samples
    Interpolated concentrations of native BDNF in human serum, and plasma samples

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).

  • Serum from ten individual healthy human male and female donors was measured in duplicate.
    Serum from ten individual healthy human male and female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.

  • Interpolated concentrations of native BDNF in rat serum sample.
    Interpolated concentrations of native BDNF in rat serum sample.

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.

  • Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.
    Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.

    L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.

  • Linearity of dilution in Sample Diluent NS.
    Linearity of dilution in Sample Diluent NS.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native BDNF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Linearity of dilution in Sample Diluent NS.
    Linearity of dilution in Sample Diluent NS.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native BDNF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Linearity of dilution in Sample Diluent NS.
    Linearity of dilution in Sample Diluent NS.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Recombinant BDNF was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

References (6)

Publishing research using ab212166? Please let us know so that we can cite the reference in this datasheet.

ab212166 has been referenced in 6 publications.

  • Tichanek F  et al. Hippocampal mitochondrial dysfunction and psychiatric-relevant behavioral deficits in spinocerebellar ataxia 1 mouse model. Sci Rep 10:5418 (2020). PubMed: 32214165
  • García-Marchena N  et al. Potential association of plasma lysophosphatidic acid (LPA) species with cognitive impairment in abstinent alcohol use disorders outpatients. Sci Rep 10:17163 (2020). PubMed: 33051508
  • Chung CC  et al. Plasma Exosomal Brain-Derived Neurotrophic Factor Correlated with the Postural Instability and Gait Disturbance-Related Motor Symptoms in Patients with Parkinson's Disease. Diagnostics (Basel) 10:N/A (2020). PubMed: 32932791
  • Wang ZH  et al. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates d-Secretase by Upregulating C/EBPß in Alzheimer's Disease. Cell Rep 28:655-669.e5 (2019). PubMed: 31315045
  • Lee HL  et al. Yuk-Mi-Jihwang-Tang , a Traditional Korean Multiple Herbal Formulae, Improves Hippocampal Memory on Scopolamine Injection-Induced Amnesia Model of C57BL/6 Mice. Evid Based Complement Alternat Med 2018:2821040 (2018). PubMed: 29507588
  • Grasman JM & Kaplan DL Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves. Sci Rep 7:4092 (2017). ELISA . PubMed: 28642578

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

ab212166 - Human BDNF ELISA Kit-Use in marmoset plasma

 Inconclusive Good 4/5 (Ease of Use)
Abreviews
abreview image
I have used the BDNF ELISA kit on proteins isolated from marmoset plasma(heparin). The samples were undiluted and diluted 1:2 and 1:4. I did not detect the BDNF in the sample of marmoset. However, I was able to detect in the same condition with the rat plasma. And, I tried to lengthen discrimination time, but was not able to detect in the plasma of the marmoset. I think that the BDNF ELISA kit ab212166 can not work for BDNF detection in marmoset sample.

Abcam user community

Verified customer

Submitted Nov 04 2016

ab212166 - Human BDNF ELISA Kit-Use in rats brain tissue

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I have used the BDNF elisa kit on proteins isolated from rat brain (hippocampus). In order to create the same conditions in both standard and samples i add 100 micro-liters of lysis buffer to the standard stock (lysis buffer- 200mM EDTA, 100mM tris,IGEPAL,Protease Inhibitor and Phosphatase Inhibitor in DDW). The samples were diluted 1:10 in order to minimize lysis buffer interference with the assay. The detection of BDNF in the samples was good as well as the standard curve which was accurate (despite the lysis buffer ). I think that the BDNF elisa kit ab212166 can defiantly work for BDNF detection in brain tissue lysats

Ravit Uzan

Verified customer

Submitted Jul 06 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com