Overview

  • Product name

    Human beta 2 Microglobulin ELISA Kit
    See all beta 2 Microglobulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.19 ng/ml
  • Range

    1 ng/ml - 20 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.


    A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.


    Get results in 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated beta 2 Microglobulin Antibody 1 x 120µl
    beta 2 Microglobulin Microplate (12 x 8 well strips) 1 unit
    beta 2 Microglobulin Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Alternative names

    • B2M
    • B2MG_HUMAN
    • Beta 2 microglobin
    • Beta 2 microglobulin
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Beta chain of MHC class I molecules
    • Beta-2-microglobulin form pI 5.3
    • CDABP0092
    • Hdcma22p
    • IMD43
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample

Protocols

References

This product has been referenced in:

  • Riega-Torres J  et al. Beta-2 Microglobulin in Whole Unstimulated Saliva Can Effectively Distinguish Between Sjögren's Syndrome and Non-Autoimmune Sicca Symptoms. Arch Rheumatol 32:284-289 (2017). Read more (PubMed: 29900998) »
  • Rizzo R  et al. Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection. J Immunol Res 2016:3890306 (2016). Read more (PubMed: 27699182) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer



We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=resource&rid=10795).

However, we do suggest the following approximate use by dates for all products:

Room temperature: 1 week

+ 4°C: 1 year

- 20°C: 5 years

- 80°C: 10 years

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Answer

Ab108885
DISCOUNT CODE: xxxxx
Expiration date:xxxxxxx

Ab99977
DISCOUNT CODE:xxxxxxxxx
Expiration date: xxxxxxxx

I am very pleased to hear that your customer would like to test ab99977 and ab108885 in human liquor. Each code will give you the value of the product off your next order before the expiration date. To redeem this offer, please submit an Abreview for human liquor and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

In addition, could you please supply me with the email address and institution of the customer so that I may complete the forms for the testing discounts?

I look forward to your reply.

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Answer

Thank you for your enquiry and your interest in our products.

Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

I would advise you to read through the datasheets as well as the Protocol Booklet (which can be found and down-loaded under the Protocol section) to see which one would suit your need:

https://www.abcam.com/beta-2-microglobulin-human-elisa-kit-1-x-96-well-plate-ab99977.html

https://www.abcam.com/beta-2-microglobulin-human-elisa-kit-ab108885.html

In Italy, we have an authorized distributor Prodotti Gianni, so if you have any further enquiry regarding shipment or order I would advise you to contact them directly. Here are their contact details:

http://www.prodottigianni.com

mailto:fberetta@prodottigianni.com

mailto:rsitta@prodottigianni.com

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

For our kits:

Regardingab99977:Unfortunately, we have not tested the capture antibody against pig Beta-2-Microglobulin; however, the detection antibody is cross reactive with porcine Beta-2-Microglobulin (B2M). This does imply high homology between porcine B2M and human B2M. Therefore, there is a good possibility that the kit will successfully react with the porcine tissue sample.

It is also important to note that this kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysate samples willlikelywork. Should you decide to test this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay DiluentBto minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined empirically.

For the original lysate, in general would want to shoot for at least 1 mg/ml total protein (preferably more) to achieve 50-500 ug/ml after dilution. Again though, this range should just be used as a starting point and the optimal concentration would need to be determined empirically.

When preparing a lysis buffer, attached are some general guidelines for doing so along with some basic tips for sample preparation. In brief, a lysis buffer must meet the following specifications:

A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain >0.1% SDS
D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)

This would include any buffers used for immunoprecipitations, including RIPA buffer.

Forab108885:We have tested porcine plasma/ serum samples with this kit. Low cross-reactivity observed so we do not suggest this kit for porcine tissue.

For our antibodies:

The Human to Porcine Identity of B2M is 72.269% overall. However there is good homology at the C terminus and in some internal regions.

Our Anti-beta 2 Microglobulin antibody [HYB332-01] (ab27155) is raised against a region with very good homology (89%). It may be found at:https://www.abcam.com/beta-2-Microglobulin-antibody-HYB332-01-ab27155.html

Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853) may be acceptable as well though as it is against the C terminus. The product page is at:https://www.abcam.com/beta-2-Microglobulin-antibody-EP2978Y-ab75853.html.

For UNTESTED species and/or applications, we have established a testing discount program. Either of these antibodies or ourbeta 2 Microglobulin Human ELISA Kit ab99977 would qualify.Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially optimised for cell culture samples and I recommend to choose them for the experiment.

2. We do offer bulk oder discounts. If you order 5 or more products you will get a discount. For 6 products you will currently get a 5% discount. Please do not forget to mention that when ordering. If you are planning to order more than 10 products, please contact our customer service department with the numbers and they might be able to offer you a higher discount.

3. I can confirm that c stands for competitive ELISA and s stands for sandwich ELISA. In a competitive ELISA the competitor is labeled with biotin and the amount of the bound competitor is measured. This means that a high OD indicates a low amount of the protein of interest in your sample. A low OD means that the competitor has been ousted and there is a high amount of the protein of interest in the sample.

4. I can confirm that the main difference between these two kits is the sensitivity. Both are suitable for cell culture supernatant, urine, serum, plasma.

Our Abpromise guarantee applies to all these kits.

https://www.abcam.com/abpromise

I hope this information is helpful and wish you good luck with your experiments. Please do not hesitate to contact me again with any further questions.

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