Key features and details
- Sensitivity: 6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman beta 2 Microglobulin ELISA Kit (B2M)
See all beta 2 Microglobulin kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 6 pg/ml
Range15.6 pg/ml - 1000 pg/ml
< 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 104.6 93% - 118% Serum 94.28 80% - 103% Plasma 95.78 81% - 105%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s beta 2 Microglobulin (B2M) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human beta 2 Microglobulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human beta 2 Microglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and beta 2 Microglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta 2 Microglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta 2 Microglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 2 x 30ml beta 2 Microglobulin Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human beta 2 Microglobulin (lyophilized) 2 vials Recombinant Human beta 2 Microglobulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in diseaseDefects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similaritiesBelongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localizationSecreted. Detected in serum and urine.
- Information by UniProt
- Beta 2 microglobin
SimpleStep ELISA kits
ab99977 has not yet been referenced specifically in any publications.