Overview

  • Product name
    Human Beta-III Tubulin Antibody Pair - BSA and Azide free
    See all beta III Tubulin kits
  • Detection method
    Colorimetric
  • Assay type
    ELISA set
  • Range
    313 pg/ml - 20000 pg/ml
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Antibody Pairs – BSA and Azide free include a capture and a detector antibody pair suitable for sandwich ELISA. The Antibody Pair can be used to quantify native and recombinant Human Beta-III Tubulin.


    For additional information on the performance of the antibody pair used in this kit, please see equivalent SimpleStep ELISA® Kit (ab235644), which use the same antibody pair. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    Please note that the range provided is only an estimation based on the performance of a related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human Beta-III Tubulin Capture Antibody 1 x 100µg
    Human Beta-III Tubulin Detector Antibody 1 x 100µg
  • Research areas
  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • Tissue specificity
    Expression is primarily restricted to central and peripheral nervous system.
  • Involvement in disease
    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • Sequence similarities
    Belongs to the tubulin family.
  • Domain
    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Alternative names
    • beta 3 tubulin
    • beta-4
    • CDCBM
    • CDCBM1
    • CFEOM3
    • CFEOM3A
    • FEOM3
    • M(beta)3
    • M(beta)6
    • MC1R
    • Neuron specific beta III Tubulin
    • Neuron-specific class III beta-tubulin
    • QccE-11995
    • QccE-15186
    • TBB3_HUMAN
    • Tubb 3
    • TUBB3
    • TUBB4
    • Tubulin beta 3
    • Tubulin beta 3 chain
    • Tubulin beta 4
    • Tubulin beta III
    • Tubulin beta-3 chain
    • Tubulin beta-4 chain
    • Tubulin beta-III
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab244131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab244131 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244131.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up