Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986)


  • Product name
    Human Beta Nerve Growth Factor ELISA Kit (NGFB)
    See all Beta nerve growth factor kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 14 pg/ml
  • Range
    6.86 pg/ml - 5000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.7 90% - 122%
    Serum 113.1 98% - 116%
    Plasma 102.8 85% - 117%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA® range.

  • Notes

    NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Beta Nerve Growth Factor Microplate (12 x 8 wells) 1 unit
    Beta Nerve Growth Factor Standard Human (recombinant) (lyophilized) 2 vials
    Biotinylated anti-Human Beta Nerve Growth Factor (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
  • Cellular localization
  • Alternative names
    • Beta nerve growth factor
    • Beta NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • Nerve growth factor (beta polypeptide)
    • Nerve growth factor beta polypeptide
    • Nerve growth factor beta subunit
    • NGFB
    • NID67
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab99986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab99986.



This product has been referenced in:
  • Shi W  et al. TrkA is a binding partner of NPM-ALK that promotes the survival of ALK+ T-cell lymphoma. Mol Oncol 11:1189-1207 (2017). Read more (PubMed: 28557340) »
  • Gilbert HT  et al. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target. Sci Rep 6:37360 (2016). ELISA ; Human . Read more (PubMed: 27853274) »

See all 5 Publications for this product

Customer reviews and Q&As

Abreview for Beta NGF Human ELISA Kit

Average Good 4/5 (Ease of Use)
The kit is easy to use,
We got a good standard curve and R2 value, however its not sensitive to the serum samples.
Some study reported that NGF level in ng/ml, but we can't detected through this kit. Why?
Could you give me the solution. Thanks!

Abcam user community

Verified customer

Submitted Jan 24 2018


Good Good 4/5 (Ease of Use)
Sample:Human Serum
We got good results: Good standard curve and R2 value, enough signal.

Abcam user community

Verified customer

Submitted Sep 14 2017

Sera of dromedary camels have been validated for the determination of b-NGF using Abcam human b-NGF ELISA kit (ab99986).
Serum from female dromedaries with suspected high serum b-NGF concentration was pooled. Then this serum was subjected to serial dilution (1:2). The dilution was carried out either in Assay Diluent (1x) provided by the manufacturer, or in pool of camel serum with low (undetectable) serum b-NGF concentration. The dilution has been done the same way as the preparation of the standard described in the protocol of the kit. Then, the assay was performed and optical density values have been plotted against dilution.
Date obtained are in the table below.
Standard Concentration Dilution Standard High CS in AD High CS in low CS
ST 1 0 8 0.08 0.07 0.07
ST 2 20.58 7 0.14 0.17 0.14
ST 3 61.7 6 0.25 0.31 0.26
ST 4 185.2 5 0.54 0.65 0.55
ST 5 555.6 4 1.25 1.35 1.22
ST 6 1667 3 2.35 2.38 2.27
ST 7 5000 2 3.32 3.2 3.15
ST 8 10000 1 3.65 3.57 3.54
In conclusion, b-NGF in camel sera after serial dilution showed an excellent parallelism compared to the standard (recombinant human b-NGF diluted in Assay Diluent). The kit is suitable for the determination of b-NGF in the serum of dromedary camels.


Abcam user community

Verified customer

Submitted Sep 13 2016

The kit is not saliva sensitive

Average Good 4/5 (Ease of Use)
The kit is easy to use,
however its not sensitive to saliva samples

Abcam user community

Verified customer

Submitted Apr 08 2015

Thank you for contacting us.

As stated on the datasheet, ab99986 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at –80&...

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Thank you for alerting us to this issue.

Do you have your original order number and lot number? Depending on which lot you received, the HRP-streptavidin may have contained 8 ul, so you should try spinning down the vial before opening and che...

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Abreview for Beta NGF Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Sample: Pig Cell (bronchioaveolar lavage)
Specification: bronchioaveolar lavage
Amount of Sample: 500 µg
Type of Kit Used: ELISA
Positive control: porcine Hippocampus
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: It is really important to follow the recommended sample and standard volumes.
Abcam response: We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.

Abcam user community

Verified customer

Submitted Aug 07 2012

Thank you for letting me know and thank you for participating in the testing program.

Please do not hesitate to let me know if there is anything I might be able to do for you in the future.

That is good news. Thank you for keeping me informed.

Please do not hesitate to contact us if you need any advice or information.

Use our products? Submit an Abreview. Earn rewards!

I am very pleased to hear you would like to accept our offer and test ab99986 in porcine. This code will give you: $470 VALUE OFF YOUR NEXT ORDERbefore the expiration date. To redeem this offer, please submit an Abreview for porcine and include this co...

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