Human beta Tubulin peptide (ab20775)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
Description
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Product name
Human beta Tubulin peptide
See all beta Tubulin proteins and peptides -
Purity
> 90 % HPLC. -
Accession
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Animal free
No -
Nature
Synthetic -
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Species
Human
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab20775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
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Form
Liquid -
Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
General Info
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Alternative names
- Beta 4 tubulin
- Beta 5 tubulin
- beta Ib tubulin
see all -
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
Images
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Western blot - Human beta Tubulin peptide (ab20775)All lanes : Anti-beta Tubulin antibody - Loading Control (ab6046) at 1/500 dilution
Lane 1 : HeLa Cell lysate
Lane 2 : A431 Cell lysate
Lane 3 : MCF7 Cell lysate
Lane 4 : 293 Cell lysate
Lane 5 : HeLa Cell lysate withHuman beta Tubulin peptide (ab20775) at 1 µg/ml
Lane 6 : A431 Cell lysate withHuman beta Tubulin peptide (ab20775) at 1 µg/ml
Lane 7 : MCF7 Cell lysate withHuman beta Tubulin peptide (ab20775) at 1 µg/ml
Lane 8 : 293 Cell lysate withHuman beta Tubulin peptide (ab20775) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab20775 has been referenced in 2 publications.
- Khan MA et al. Adenine overload induces ferroptosis in human primary proximal tubular epithelial cells. Cell Death Dis 13:104 (2022). PubMed: 35110539
- Zhao H et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology 153:2701-13 (2012). PubMed: 22508516
