Overview

  • Product name

    Human Betacellulin ELISA Kit
    See all Betacellulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5%
  • Sample type

    Cell culture supernatant, Milk, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    15 pg/ml
  • Range

    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 83 82% - 85%
    Serum 104 103% - 106%
    Tissue Culture Media 101 99% - 103%
    Heparin Plasma 99 98% - 101%
    EDTA Plasma 90 87% - 92%
    Citrate Plasma 94 91% - 100%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Betacellulin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Betacellulin protein in Human serum, plasma and milk samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Betacellulin (BTC) is an EGF growth factor family member that binds to EGF Receptor family members. BTC is expressed as an 178 amino acid precursor form that is a single-pass transmembrane protein (this form is called probetacellulin). Subsequent cleavage produces the soluble and secreted 80 amino acid betacellulin fragment from the extracellular portion of probetacellulin. This assay reacts with the soluble betacellulin protein. There is high BTC protein sequence similarity among mammals.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human BTC Capture Antibody 1 x 600µl
    10X Human BTC Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human BTC Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
  • Cellular localization

    Cell Membrane and Secreted; extracellular space.
  • Alternative names

    • Betacellulin precursor
    • BTC
    • Probetacellulin
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab189575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Serum from 10 apparently healthy male donors was measured in duplicate. Six of the donors tested below the lowest tested standard. The mean Betacellulin concentration of the four donors was determined to be 125 pg/mL with a range of 88-173 pg/mL.

Protocols

References

ab189575 has not yet been referenced specifically in any publications.

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