Overview

  • Product name

    Human Betacellulin ELISA Kit (BTC)
    See all Betacellulin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    24.69 pg/ml - 18000 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.34 65% - 85%
    Plasma 78.86 67% - 88%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Human
  • Product overview

    Abcam’s Betacellulin (BTC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Betacellulin in plasma, and cell culture supernatants. (Human Betacellulin concentration is pretty low in normal plasma, it may not be detected in this assay). 


    This assay employs an antibody specific for Human Betacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Betacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Betacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Betacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human Betacellulin in normal serum with ab99987, only in serum spiked with Human Betacellulin.


    Get higher sensitivity in only 90 minutes with Human Betacellulin ELISA Kit (ab189575) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    240X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Betacellulin Microplate (12 x 8 well strips) 1 unit
    Betacellulin Standard Human (recombinant) (lyophilized) 2 vials
    Biotinylated anti-Human Betacellulin (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
  • Cellular localization

    Cell Membrane and Secreted; extracellular space.
  • Alternative names

    • Betacellulin precursor
    • BTC
    • Probetacellulin
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab99987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99987.

Protocols

References

This product has been referenced in:

  • Ivars M  et al. The involvement of ADAM10 in acantholysis in mucocutaneous pemphigus vulgaris depends on the autoantibody profile of each patient. Br J Dermatol N/A:N/A (2019). Read more (PubMed: 31370093) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

BTC crossi reactivity in mouse brains

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We have shown that "betacellulin" can been detected in mouse brain homogenate (using the human BTC elisa kit)with levels in the striatum and frontal cortex of 1879 ng/ul and 2311 ng/ul respectively.

Dr. Rejhan Idrizi

Verified customer

Submitted Oct 18 2013

Answer

Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacellulin). The most cost effective approach for you might be an indirect ELISA, which requires coating the sample onto the plate, then detecting with an antibody specific for the protein of interest and then detecting that with an anti-IgG secondary antibody conjugated to an enzyme such as HRP. Please let me know if you have any questions.

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Question
Answer

We have not tested human plasma/serum concentration but the following publication does mention that 40ng/ml (40,000pg/ml) concentration is found in human plasma.



Betacellulin Induces Increased Retinal Vascular Permeability in Mice.

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013444


Fig S2: No significant increase in circulating plasma betacellulin in patients with proliferative diabetic retinopathy. Plasma was prepared from whole blood from patients with no diabetes, diabetes with no evidence of retinopathy and proliferative diabetic retinopathy and analyzed by ELISA for betacellulin.


Since the standard range is 10pg/ml˜100,000pg/ml, you may want to dilute your sample 1:10˜1:1000 to start with. Please bear in mind that this is a starting concentration and the optimum concentration/dilution needs to be determine by the researcher.

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Answer

Thank you for contacting us.

I am very sorry to hear these kits failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I’d like to gather further information. I would really appreciate if you could please clarify the following:

- What are the lot numbers for both kits and when where they received? (I would very much appreciate if you could please provide the order number as well).

- How were the kits stored and for how long? And how were the standards stored after reconstitution?

- Did you follow the standard preparation guidelines mentioned in the kit protocols?

I am happy to investigate this case for you, and the event that the kits sent happened to be faulty, I will replace o reimburse it as stated by the Abpromise guarantee.

I look forward to receiving your reply. Have a nice day.

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Answer

Thank you for contacting us.

Unfortunately wearenot able to find a publication stating what the normal levels of betacellulin in human serum/plasma was either. Based on our QC tests on the kit with normal human serum and plasma samples, the measured levels were below the sensitivity limit (30 pg/ml) which means they were undetectable. Therefore, this kit would likely be most useful testing diseased or treated serum/plasma samples with expected elevated levels of betacellulin than normal.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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